We narrowed to 8,907 results for: Ott
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Plasmid#75126PurposeRetroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domainDepositorInsertKMT6 (EZH2 Human)
UseRetroviralTagsNoneMutationSET domain replaced with the SET domain of EZH1Available SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain o…Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W
Plasmid#163177PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tagDepositorAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4(TA) + PGK-blast
Plasmid#167827PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
Tagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-blast
Plasmid#167820Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
Tagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W
Plasmid#163178PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-puro
Plasmid#167824PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
Tagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1 124A/125A
Plasmid#164523PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)DepositorInsertNSP1 124A/125A (ORF1ab )
ExpressionMammalianMutationInsert of NSP1 gene's CDS from SARS-CoV-2 is…Available SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)
Plasmid#197505PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…PromoterCMV and T7Available SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucAL
Plasmid#174049PurposeExpresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinaseDepositorInsertLucAL
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationALG8 alanine 506 to threonine; LAMA4 glycine 1254…PromoterHuman Ubiquitin CAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-PARK7WT-VA
Plasmid#115182PurposeLentiviral transduction and expression of PARK7WT into any mammalian cellDepositorInsertPARK7WT (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4(TA)-ires-puro
Plasmid#167830PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS1401
Plasmid#29198PurposeHigh copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporterDepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
ACTIN-Cxcl5-PGK-Cre
Plasmid#110278PurposeBicistronic lentivirus expressing Cxcl5 and CreDepositorInsertsUseCre/Lox and LentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterB-actin and PgkAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-blast
Plasmid#167825PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
Tagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-C20orf24- C20orf24-3’UTR-WT
Plasmid#128508PurposeLentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.DepositorAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLV1 L2_pECIA2
Plasmid#115100PurposeBait vector CLV1 L2_pECIA2 should be used with prey vector CLV1 L2_pECIA14.DepositorInsertAT1G75820 (CLV1 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
GSO2/EDA23 X079_pECIA2
Plasmid#115111PurposeBait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.DepositorInsertAT5G44700 (GSO2 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
BRI1 BRI1_pECIA2
Plasmid#115086PurposeBait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.DepositorInsertAT4G39400 (BRI1 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RPK2 X042_pECIA2
Plasmid#115135PurposeBait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.DepositorInsertAT3G02130 (RPK2 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK1 A6_pECIA2
Plasmid#114967PurposeBait vector SERK1 A6_pECIA2 should be used with prey vector SERK1 A6_pECIA14.DepositorInsertAT1G71830 (SERK1 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLV1 L2_pECIA14
Plasmid#114900PurposePrey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK3/BAK1 BAKNcFus_pECIA14
Plasmid#114775PurposePrey vector SERK3/BAK1 BAKNcFus_pECIA14 should be used with bait vector SERK3/BAK1 BAKNcFus_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK1 A6_pECIA14
Plasmid#114767PurposePrey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1538
Plasmid#29261PurposeHigh copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporterDepositorInsertPle67 (FEV Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable SinceOct. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-FLEX-CAG_G-PTEN
Plasmid#227438PurposeAn AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.DepositorInsertG-PTEN sensor (Pten Rat)
UseAAVTagsCD-sREACh and mEGFPExpressionMammalianMutationR14GPromoterpCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)
Plasmid#223110PurposepCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpG-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)
Plasmid#223111PurposepCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianPromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-P2A-EGFP (MSP2582)
Plasmid#223067PurposepCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFPDepositorInserthuman codon optimized SpCas9-P2A-EGFP
UseCRISPRTagsNLS(SV40)-3xFLAG-P2A-EGFPExpressionMammalianPromoterCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)
Plasmid#231233PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
ExpressionMammalianMutationTAD deleted (delta1-143); numbering based on sequ…Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-MycΔdeg1∆deg2
Plasmid#231243PurposeHA-tagged MYC over-expession construct with point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cellsDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianPromoterTroponin T promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCRbluntII-pHES7-STOPgRNA
Plasmid#204349PurposegRNA to target pig HES7 stop codonDepositorInsertPig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
ExpressionMammalianAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN
Plasmid#172435PurposeLentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKLDepositorInsertsCre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer 21-3xFlag-hMEFV-F479L
Plasmid#195477PurposepInducer21 plasmid containing the human MEFV gene with the F479L mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationchanged phenylalanine 479 to leucineAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer 21-3xFlag-hMEFV-ΔPYD
Plasmid#195478PurposepInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationdeletion of residues 1-92 of pyrin (the PYD domai…Available SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer21-3xFlag-hMEFV-Q426R
Plasmid#195475PurposepInducer21 plasmid containing the human MEFV gene with the Q426R mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationchanged glutamine 426 to arginineAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W
Plasmid#163147PurposeLentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tagDepositorAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W
Plasmid#163175PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W
Plasmid#163174PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only