We narrowed to 3,228 results for: RFP

1. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLM1110_LEU2

   Plasmid

   #168460

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   mRFP1
   

   Use

   Synthetic Biology; Yeast assembly vector for big-…

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

   Plasmid

   #218509

   Purpose

   integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mKate2
   

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Deletion of Plac_mrfp cassette, introduction of a…

   Promoter

   P23

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pINDUCER12 (miR-RUL)

   Plasmid

   #46935

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MultiMate 5 colors

   Plasmid

   #206266

   Purpose

   Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
   

   Use

   Recombinant baculovirus production, cre acceptor…

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOTTC414 - pAAV EF1a hPREP

   Plasmid

   #59967

   Purpose

   An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST_6xUAS:Lamp1-mGFP

   Plasmid

   #192356

   Purpose

   Lamp1 tagged with mGFP, expressed under the UAS promoter

   Depositor

   Francesca Peri

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   Lamp1 (LAMP1 Human)
   

   Tags

   mGFP

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

    Plasmid

    #135564

    Purpose

    An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 FF3
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    SYN1 and hSYN1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63884

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

    Plasmid

    #204622

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    mRFP1
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

    Plasmid

    #105020

    Purpose

    Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Znrf1 (Znrf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

    Plasmid

    #121538

    Purpose

    An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

    Depositor

    Christopher Richie

    Article

    Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

    Has Service

    AAV2 and AAV5

    Insert

    HA-tagged hM4D(Gi) (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SYN1

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FU-mILa-CRW

    Plasmid

    #84556

    Purpose

    lentiviral expression of mouse IL1a and mRFP

    Depositor

    Li Xin

    Article

    Zhang et al Mol Cell. 2016 Sep 15;63(6):976-89. doi:

    Insert

    IL1a (Il1a Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge