We narrowed to 6,087 results for: nls

1. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

   Plasmid

   #101182

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

   Plasmid

   #101183

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   M694A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

   Plasmid

   #101184

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mutation

   G582A/V583A/E584A/D585A/N588A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

   Plasmid

   #101179

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

   Plasmid

   #101180

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RV-cag-Dio-Kif5c-2A-tdt

   Plasmid

   #87668

   Purpose

   retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

   Depositor

   Oscar Marin

   Article

   Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

   Insert

   Kif5C-P2A-mTdt (Kif5c Rat)
   

   Use

   Retroviral

   Tags

   palmitylation

   Expression

   Mutation

   motor domain aa1-560 present, V40A, E125V (see de…

   Promoter

   CAG

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAN1646

   Plasmid

   #127205

   Purpose

   pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-hCas9D10A

    Plasmid

    #52101

    Purpose

    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

    Depositor

    Izuho Hatada

    Insert

    hCas9D10A
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pX330-Flag-eSpCas9-D10A

    Plasmid

    #80449

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_D10A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-Flag-eSpCas9-H840A

    Plasmid

    #80454

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-Flag-HFSpCas9-H840A

    Plasmid

    #80455

    Purpose

    high fidelity SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRha-ABE8e-NRCH

    Plasmid

    #165417

    Purpose

    For bacterial purification of ABE8e-NRCH protein

    Depositor

    David Liu

    Insert

    ABE8e-NRCH
    

    Use

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-ABE8e-NRCH

    Plasmid

    #165416

    Purpose

    Expresses the base editor ABE8e-NRCH in mammalian cells

    Depositor

    David Liu

    Insert

    ABE8e-NRCH
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

    Plasmid

    #79785

    Purpose

    genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

    Depositor

    Anthony Zador

    Insert

    MAPP-nL
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

    Plasmid

    #129111

    Purpose

    Homologous recombination vector for excision reporter 1 in the CLYBL locus.

    Depositor

    Bruce Conklin

    Insert

    LSL, eGFP, NLS-RFP
    

    Use

    Cre/Lox and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-Flag-HeFSpCas9-H840A

    Plasmid

    #80456

    Purpose

    high fidelity enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HeFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge