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We narrowed to 18,567 results for: puro

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Showing: 3181 - 3200 of 18567 results

  1. pCSIIpuro-AREG-ScNeo

    Plasmid
    #209897
    Purpose
    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Publication
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    AREG-ScNeo (AREG Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209897-plasmid-map-sequence-id-416601

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  3. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid
    #136405
    Purpose
    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.
    Depositor
    Massimo Squatrito
    Publication
    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    BbsI restriction site at position 437 was removed…
    Promoter
    U6, PDK
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136405-plasmid-map-sequence-id-269877

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  7. pCW57.1 Lysosomal-METRIQ

    Plasmid
    #135401
    Purpose
    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
    Depositor
    Eisuke Itakura
    Publication
    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10
    Insert
    DNAse II alpha (DNASE2 Human)
    Use
    Lentiviral
    Tags
    T2A, mCherry, and sfGFP
    Expression
    Mammalian
    Mutation
    Single-nucleotide polymorphism C432T
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    135401-plasmid-map-sequence-id-273129

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  9. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    113193-plasmid-map-sequence-id-216317

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  10. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    91565-plasmid-map-sequence-id-456463

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  14. pSBbi-BP

    Plasmid
    #60512
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    60512-plasmid-map-sequence-id-97534

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  18. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    139086-plasmid-map-sequence-id-273882

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Showing: 3181 - 3200 of 18567 results