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pGDP1 NDM-1

Plasmid

#112883

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

NDM-1 (blaNDM-1 Klebsiella pneumoniae)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly

Plasmid

#211355

Purpose

Transient expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB-wt

Plasmid

#58905

Purpose

mammalian expression of wild type human Rb

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTW2657

Plasmid

#252577

Purpose

TRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtPDS3 gRNA 12 and AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET32a-hTGFa

Plasmid

#199236

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Transforming growth factor alpha, partial (TGFA Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Prolactin-CD4d3+4-bio

Plasmid

#73123

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PRL (PRL Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTW2154

Plasmid

#236578

Purpose

ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDOXOFF 15xGly

Plasmid

#211345

Purpose

DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTW2066

Plasmid

#236575

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hysn-flex-dsRed-shscramble

Plasmid

#71383

Purpose

Expresses scramble shRNA Cre-dependently by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

human synapsin promoter-flex switch-dsRed-shscramble

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK

Plasmid

#29689

Purpose

GFP reporter protein fused to three GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site

Promoter

SP6

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCGT001_nuclear-mOrange2

Plasmid

#248083

Purpose

Constitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mOrange2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMK025

Plasmid

#236572

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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PB-fLuc=tdT

Plasmid

#120870

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-fLuc=tdT

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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