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Showing: 6401 - 6440 of 8371 results

  1. pExo1

    Plasmid
    #53357
    Purpose
    Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.
    Depositor
    Lingchong You
    Article
    Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10
    Inserts
    BlaMs
    hlyAs
    Use
    Tags
    Expression
    Mutation
    Promoter
    p(lac/ara-1)
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    53357-plasmid-map-sequence-id-83079

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  2. mEmerald-ERBB2-N-18

    Plasmid
    #62755
    Purpose
    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    ERBB2 (ERBB2 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62755-plasmid-map-sequence-id-406429

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  3. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  5. HSP90B1

    Plasmid
    #39076
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSP90B1 (HSP90B1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pACP-ADRβ2 Control Plasmid

    Plasmid
    #101128
    Purpose
    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Insert
    Beta-2-Adrenergic Receptor (ADRB2 Human)
    Use
    Tags
    ACP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101128-plasmid-map-sequence-id-194168

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  8. Lamin A (R298C)-mRFP

    Plasmid
    #124269
    Purpose
    Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R298C) (LMNA Human)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R298C)
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124269-plasmid-map-sequence-id-241969

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  10. HSD17B11

    Plasmid
    #39159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B11 (HSD17B11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. huntingtin_V2095-V3138_CbioCflag

    Plasmid
    #194764
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194764-plasmid-map-sequence-id-402574

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  12. huntingtin_T97-M2069_NbioCflag

    Plasmid
    #194763
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194763-plasmid-map-sequence-id-402570

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  13. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid
    #85398
    Purpose
    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    S27A
    Promoter
    human Synapsin1 promotor and ribosomal skip seque…
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    85398-plasmid-map-sequence-id-201308

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  15. pDONR221_SMAD2_HAe2_Bsd

    Plasmid
    #167166
    Purpose
    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
    Depositor
    Alexander Loewer
    Insert
    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    Use
    Repair template for gene editing in human cells
    Tags
    blasticidin
    Expression
    Mutation
    Promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167166-plasmid-map-sequence-id-330264

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  17. MAP3K3

    Plasmid
    #38937
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAP3K3 (MAPK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. Hs.KRAS4a G13D

    Plasmid
    #83170
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G13D
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83170-plasmid-map-sequence-id-158133

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  19. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 13, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    209733-plasmid-map-sequence-id-414202

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  20. SDC229-B03

    Plasmid
    #58763
    Purpose
    bacterial expression of PDB 4PYZ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PYZ (USP7 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid
    #90243
    Purpose
    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90243-plasmid-map-sequence-id-178297

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  22. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

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  23. human KANK1 Ankyrin Repeats pET151

    Plasmid
    #164053
    Purpose
    Expresses human KANK1 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164053-plasmid-map-sequence-id-327672

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  24. MS2_SC2_S_Omicron

    Plasmid
    #180229
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Omicron (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180229-plasmid-map-sequence-id-352514

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  25. MS2_SC2_S_WT

    Plasmid
    #175044
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175044-plasmid-map-sequence-id-343940

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  26. AKT3

    Plasmid
    #39038
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKT3 (AKT3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. Syn-ChR-tdTomato

    Plasmid
    #99843
    Purpose
    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter
    Depositor
    Gordon Shepherd
    Insert
    ChR2(H134R)-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    ChR2(H134R), carrying a single point mutation at …
    Promoter
    human synapsin I promoter
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    99843-plasmid-map-sequence-id-202528

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  30. CYP7A1 (3SN5)

    Plasmid
    #32874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP7A1 (CYP7A1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amino acids 25-503 only
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. WDR5

    Plasmid
    #36900
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    mutation version of plasmid #60121 3UR4
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  36. Hs.KRAS4a Q61L

    Plasmid
    #83171
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83171-plasmid-map-sequence-id-158134

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  37. LMNB2

    Plasmid
    #36896
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LMNB2 (LMNB2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Laminb2 Ig-like domain, amino acid resid…
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. Hs.KRAS4b A146T

    Plasmid
    #83150
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    A146T
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83150-plasmid-map-sequence-id-158113

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  39. MAT2B

    Plasmid
    #39080
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT2B (MAT2B Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Gian Luca Forni, Marco Musso
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

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Showing: 6401 - 6440 of 8371 results