We narrowed to 44,081 results for: cha

1. pMC-9-35S-11

   Plasmid

   #197759

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   35S terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJMC-2-Reverse

   Plasmid

   #197801

   Purpose

   Level 1 Module 2 destination vector (reverse orientation MoClo insert)

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCZGY553

   Plasmid

   #110883

   Purpose

   Pmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neurons

   Depositor

   Andrew Chisholm

   Article

   Ghosh-Roy et al J Neurosci. 2010 Mar 3;30(9):3175-83. do

   Insert

   Pmec-4
   

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBzCas13b

   Plasmid

   #164862

   Purpose

   Inducible expression of BzCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   Bergeyella zoohelcum Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSETT4tip

   Plasmid

   #153414

   Purpose

   φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

   Depositor

   Joern Kalinowski

   Article

   Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tipA promoter from Streptomyces lividans

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4046437

   Plasmid

   #145415

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046431

   Plasmid

   #145526

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. EGFP-PML peptide

   Plasmid

   #175246

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pISH-Neurod1

   Plasmid

   #74396

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

   Insert

   neurogenic differentiation 1 (Neurod1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRPML2-CFP

    Plasmid

    #18831

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pJC45BS104 Y819W

    Plasmid

    #1307

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 Y819W (HSP104 Budding Yeast)
    

    Use

    Tags

    10X His tag

    Expression

    Bacterial

    Mutation

    Changed Tyr 819 to Trp

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046422

    Plasmid

    #145515

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UbG75C

    Plasmid

    #37194

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    Ubiquitin G75C mutant (RBX1 Human)
    

    Use

    Tags

    No tag

    Expression

    Bacterial

    Mutation

    Glycine 75 changed to Cystine

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pTXG-hTDG

    Plasmid

    #52281

    Purpose

    Bacterial expression of human TDG C-terminally fused to GyrA intein and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Use

    Tags

    GyrA-intein-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046173

    Plasmid

    #149126

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pBS-FAK

    Plasmid

    #74478

    Purpose

    This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

    Depositor

    J Thomas Parsons

    Article

    Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

    Insert

    FAK (PTK2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJEC578

    Plasmid

    #174366

    Purpose

    dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    dCas9 fused to SYNZIP18
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23150

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pISH-Neurog1

    Plasmid

    #74395

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

    Insert

    neurogenin 1 (Neurog1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046397

    Plasmid

    #145479

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pHu6-gRNA-NT1

    Plasmid

    #81203

    Purpose

    hU6 expression of gRNA NT1

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA NT1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLNL1133

    Plasmid

    #160864

    Purpose

    PJ23110-RBS-ScEEB1-mRuby2-tL3S2P24_KanR_p15A

    Depositor

    John Dueber

    Article

    Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

    Insert

    Medium-chain fatty acid ethyl ester synthase
    

    Use

    Synthetic Biology

    Tags

    mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23110

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046506

    Plasmid

    #145560

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046486

    Plasmid

    #145506

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046351

    Plasmid

    #145449

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pRoTnsABCDQ

    Plasmid

    #178772

    Purpose

    Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTXG-hXRCC1

    Plasmid

    #52283

    Purpose

    Bacterial expression of human XRCC1 C-terminally fused to GyrA intein and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GyrA-intein-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBW-m4046217

    Plasmid

    #149071

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046581

    Plasmid

    #149064

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pGBW-m4046589

    Plasmid

    #145450

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pHFX-cas3UD

    Plasmid

    #174385

    Purpose

    Plasmids for gene knockout were constructed based on suicide plasmid pHFX

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    Cas3UD
    

    Use

    Archaeal gene knockout vector for haloferax medit…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGBW-m4046249

    Plasmid

    #148991

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pGBW-m4046268

    Plasmid

    #145423

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pGBW-m4046216

    Plasmid

    #145490

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA5/FRT/TO GFP DNAK

    Plasmid

    #19490

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    dnaK molecular chaperone DnaK
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDS255

    Plasmid

    #34970

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMDC32-SEIPIN3

    Plasmid

    #96975

    Purpose

    Express Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLsCas13a-gRNA-1

    Plasmid

    #164865

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB8382

    Plasmid

    #126916

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGBW-m4046380

    Plasmid

    #148977

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. MDH1-miR-181a1-H2Kb2

    Plasmid

    #17847

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    H-2Kb (H2-K1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pfMDH_H303A

    Plasmid

    #67730

    Purpose

    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pfMDH_D230A

    Plasmid

    #67725

    Purpose

    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed aspartate 230 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pfMDH_N191A

    Plasmid

    #67724

    Purpose

    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 191 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pfMDH_K295A

    Plasmid

    #67726

    Purpose

    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 295 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

47. pRS406-GFP-SNC1(L96V)

    Plasmid

    #105278

    Purpose

    Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Snc1 (SNC1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Leucine 96 changed to Valine, intron removed

    Promoter

    TPI1

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCS2 xRor2-EGFP

    Plasmid

    #67163

    Purpose

    mammalian expression / in vitro transcription

    Depositor

    Alexandra Schambony

    Article

    Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

    Insert

    Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
    

    Use

    In vitro transcription

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pDS247

    Plasmid

    #34967

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, V416I, T–2S

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDS248

    Plasmid

    #34968

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, V416I

    Promoter

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge