We narrowed to 17,090 results for: EMB

1. pTH753-CEN-RLuc/min346maxCFLuc

   Plasmid

   #38219

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 346 codons of the original FLuc gene we…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH747-CEN-RLuc/min4maxCFLuc

   Plasmid

   #38213

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first four codons of the original FLuc gene w…

   Promoter

   ADH1 and TDH3 (=GDP)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CCR5-SZ218a QTY

   Plasmid

   #162444

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-C chemokine receptor type 5 (CCR5 Human)
   

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Availability

   Academic Institutions and Nonprofits only

   

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4. CCR5-SZ190b QTY

   Plasmid

   #162445

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-C chemokine receptor type 5 (CCR5 Human)
   

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-ZZ-TEV-SNAPf-DYNC2H1

   Plasmid

   #132524

   Purpose

   Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNC2H1 (DYNC2H1 Human)
   

   Tags

   8x His, Linker-TEV-linker-TEV, Precission cleavag…

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

   Plasmid

   #102421

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 (Tet1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Modified at the Dharmacon SMARTpool siRNA #2 targ…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IP-EGFP-PA-BAX(mito)

   Plasmid

   #228942

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-nAChr-Alpha6-GFP

   Plasmid

   #50486

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   GFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the GFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3.1-nAChr-beta3-YFP

   Plasmid

   #50484

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP fusion in M3-M4 loop (after residue P379). O…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

10. EGFP-INPP5E-CAAX

    Plasmid

    #202746

    Purpose

    express plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T1-TEV-HsFbxo7-129-398/Skp1

    Plasmid

    #202592

    Purpose

    Bacterial expression of HsFbxo7-129-398 and HsSkp1

    Depositor

    Brenda Schulman

    Article

    Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

    Inserts

    Fbxo7 (FBXO7 Human)
    Skp1 (SKP1 Human)
    

    Tags

    GST, TEV

    Expression

    Bacterial

    Mutation

    aa 129-398 only

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

    Plasmid

    #192241

    Purpose

    knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid

    #105803

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-FLAG; ORF2: TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid

    #50483

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    YFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the YFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-nAChr-beta3-CFP

    Plasmid

    #50485

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. MSCV-KRAS-G12R-IRES-mCherry

    Plasmid

    #221023

    Purpose

    Fluorescent reporter for expressing the KRAS G12R mutant CDS

    Depositor

    Mohamed Fareh

    Article

    Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

    Insert

    KRAS G12R (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-KRAS-G12S-IRES-mCherry

    Plasmid

    #221024

    Purpose

    Fluorescent reporter for expressing the KRAS G12S mutant CDS

    Depositor

    Mohamed Fareh

    Article

    Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

    Insert

    KRAS G12S (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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