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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 CA-LRP6-GFP

Plasmid

#29682

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

LDL-receptor related protein 6 (LRP6 Human)

Tags

GFP

Expression

Mammalian

Mutation

extracellular region deleted

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag7

Plasmid

#169330

Purpose

Expression of TOMM20-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag7 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hysn-flex-dsRed-shscramble

Plasmid

#71383

Purpose

Expresses scramble shRNA Cre-dependently by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

human synapsin promoter-flex switch-dsRed-shscramble

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK

Plasmid

#29689

Purpose

GFP reporter protein fused to three GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site

Promoter

SP6

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT1

Plasmid

#106304

Purpose

Express SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-fDIO-mNeonGreen

Plasmid

#241240

Purpose

FRT-dependent expression of fluorescent protein, mNeonGreen, in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

fDIO-mNeonGreen

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDF0039 DisCas7-11 only expression with CRISPR array

Plasmid

#172500

Purpose

Encodes the DisCas7-11 protein only for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 only expression with CRISPR array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT2SCb

Plasmid

#59385

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat2

Use

Template

Mutation

The 3’-end of the chloramphenicol resistance gene…

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169317

Purpose

Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightG

Plasmid

#217656

Purpose

Expresses norepinephrine indicator nLightG under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightG

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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C8orf55-CD4d3+4-bio

Plasmid

#73126

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C8orf55 (THEM6 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

Plasmid

#226517

Purpose

Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

CalR-SNAP-tag2-KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eLACCO1.1

Plasmid

#168789

Purpose

Fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

CAG

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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