We narrowed to 19,138 results for: Tat

Showing: 3201 - 3220 of 19138 results

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hSyn ArcLightCo (Q239)-T2A-nls-mCherry

Plasmid

#85844

Purpose

Genetically encoded voltage sensor ArcLight- codon optimized

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

ArcLightCo-Q239

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

hSyn1

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-EF1a-mTagRFP-T-CAAX

Plasmid

#167973

Purpose

AAVS1 targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR; Donor template

Tags

mTagRFP-T-CAAX

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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sgKRAS(A)

Plasmid

#85566

Purpose

Expresses sgRNA targeting human KRAS around codon G12

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

KRAS proto-oncogene, GTPase (KRAS Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
ABE7.10-F148A

Plasmid

#132946

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

ABE7.10(F148A)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

ABE7.10(F148A)

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pOz-humBirA-GFP

Plasmid

#133283

Purpose

Expression of BirA-GFP fusions in mammalian cells

Depositor

Arman Kulyyassov

Article

Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

Insert

eGFP

Use

Retroviral

Tags

Humanized wild type biotin ligase BirA

Expression

Mammalian

Promoter

MoMuLV

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

Plasmid

#123362

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

Firefly luciferase

Use

CRISPR, Lentiviral, and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-PKCa-mEGFP-N1

Plasmid

#112269

Purpose

IDOCKSalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

Protein Kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTECH-MmAcK3RS(IPYE)

Plasmid

#104071

Purpose

Expression plasmid encoding MmAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmAcK3RS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Axin1

Plasmid

#162543

Purpose

sgRNA targeting Axin1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Axin1 (Axin1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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GLDC-RES

Plasmid

#72882

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

GLDC (GLDC Human)

Use

Lentiviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shGLDC_2

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FLEX-rc[Chronos-GFP]

Plasmid

#62725

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) HIPK3 Sense

Plasmid

#60634

Purpose

Expresses the human HIPK3 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Homeodomain-interacting protein kinase 3 (HIPK3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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