We narrowed to 14,579 results for: pET

1. pET29_anti-MERS-Nanobody-VHH-1

   Plasmid

   #206890

   Purpose

   Express anti-MERS-Nanobody-VHH-1 in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   anti-mers-nanobody-vhh-1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pET21-pelB-LaG94-1--LaG94-14

   Plasmid

   #228973

   Purpose

   For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-1--LaG94-14
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b-SUMO-FLAG-TeKaiC

   Plasmid

   #229222

   Purpose

   For expression of T. elongatus KaiC protein

   Depositor

   Andy LiWang

   Insert

   kaiC (kaiC T. elongatus)
   

   Use

   Tags

   SUMO-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b-ULP1-405-621

   Plasmid

   #229224

   Purpose

   For expression of ULP1 protein

   Depositor

   Andy LiWang

   Insert

   ULP1 (ULP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   405-621 (truncated)

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-SUMO-SeKaiB-FLAG

   Plasmid

   #229219

   Purpose

   For expression of S. elongatus KaiB protein

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB S. elongatus)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-6xHis-GFP(-7)-neg_tag1

   Plasmid

   #219419

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   GFP(-7)-neg_tag1
   

   Use

   Tags

   DEEEDD and MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Use

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-LSSmGFP-TEV-mScarlet-i

    Plasmid

    #191199

    Purpose

    Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-LSSmGFP-TEV-SAV

    Plasmid

    #191200

    Purpose

    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-T5-MfnG-TEV-His

    Plasmid

    #191114

    Purpose

    Expresses MfnG-TEV-His in E. coli

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-PKP3(305-797)

    Plasmid

    #202599

    Purpose

    Expresses ARM domain of plakophilin3

    Depositor

    Tina Izard

    Article

    Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

    Insert

    plakophilin3 (PKP3 Human)
    

    Use

    Tags

    His8 tag with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1 to 304

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-a(+)-6xHis-miRFP(-5)-HO1

    Plasmid

    #219424

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Inserts

    miRFP682
    HO1
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Contains Ribosomal Binding Site for expression of…

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-NPR4 (SA binding domain)

    Plasmid

    #226885

    Purpose

    Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

    Depositor

    Ning Zheng

    Article

    Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

    Insert

    The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    NPR4 SA-binding core (residues 373-516), with a s…

    Promoter

    T7

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a HsDHFR-bio-10His

    Plasmid

    #193883

    Purpose

    Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence

    Depositor

    James Inglese

    Article

    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

    Insert

    human dihydrofolate reductase (DHFR) (DHFR Human)
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-UBE3A iso 2 N terminus

    Plasmid

    #185643

    Purpose

    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells

    Depositor

    Irina Bezsonova

    Insert

    ubiquitin-protein ligase E3A (UBE3A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptetO

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge