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Plasmid#147705PurposeInsect Expression of DmNot3-del723-745-dsRNAresDepositorInsertDmNot3-del723-745-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN3-L676EG683E-del460-465_U
Plasmid#147658PurposeInsect Expression of DmPAN3-L676EG683E-del460-465DepositorInsertDmPAN3-L676EG683E-del460-465 (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T_1-58-del28-43_T
Plasmid#147596PurposeInsect Expression of Dm4E-T_1-58-del28-43DepositorInsertDm4E-T_1-58-del28-43 (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del380-404-W507E_S
Plasmid#147499PurposeMammalian Expression of HsNot2-del380-404-W507EDepositorInsertHsNot2-del380-404-W507E (CNOT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3-del607-634-F673E_S
Plasmid#147503PurposeMammalian Expression of HsNot3-del607-634-F673EDepositorInsertHsNot3-del607-634-F673E (CNOT3 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_862-1384_R
Plasmid#147392PurposeInsect Expression of CeAIN2_254-400-DmGW182_862-1384DepositorInsertCeAIN2_254-400-DmGW182_862-1384 (gw Nematode, Fly)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R
Plasmid#147393PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690DepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690 (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-del205-490-dsRNAres_R
Plasmid#147402PurposeInsect Expression of DmGW182-del205-490-dsRNAresDepositorInsertDmGW182-del205-490-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384_R
Plasmid#147384PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384DepositorInsertCeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-del539-830-dsRNAres_O
Plasmid#147153PurposeInsect Expression of DmGW182-del539-830-dsRNAresDepositorInsertDmGW182-del539-830-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTNRC6C_1381-1690-del1419-1510_M
Plasmid#146945PurposeMammalian Expression of HsTNRC6C_1381-1690-del1419-1510DepositorInsertHsTNRC6C_1381-1690-del1419-1510 (TNRC6C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-MGW-RRM-Cterm_H
Plasmid#146485PurposeInsect Expression of DmGW182-MGW-RRM-CtermDepositorInsertDmGW182-MGW-RRM-Cterm (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM2-delDUF-delCterm_H
Plasmid#146480PurposeInsect Expression of DmGW182-delM2-delDUF-delCtermDepositorInsertDmGW182-delM2-delDUF-delCterm (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L
Plasmid#146838PurposeInsect Expression of DmGW182-PAM26B-F1370A-dsRNAresDepositorInsertDmGW182-PAM26B-F1370A-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-SD6B-F13170A-dsRNAres_L
Plasmid#146841PurposeInsect Expression of DmGW182-SD6B-F1370A-dsRNAresDepositorInsertDmGW182-SD6B-F1370A-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6Biso1_1-1619-del1395-1534_L
Plasmid#146849PurposeInsect Expression of HsTNRC6Biso1_1-1619-del1395-1534DepositorInsertHsTNRC6Biso1_1-1619-del1395-1534 (TNRC6B Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1199-delDUF-M2-dsRNAres_L
Plasmid#146821PurposeInsect Expression of DmGW182_1-1199-delDUF-M2-dsRNAresDepositorInsertDmGW182_1-1199-delDUF-M2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delCterm-delDUF-dsRNAres_L
Plasmid#146827PurposeInsect Expression of DmGW182-delCterm-delDUF-dsRNAresDepositorInsertDmGW182-delCterm-delDUF-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delCterm-delM2-dsRNAres_L
Plasmid#146828PurposeInsect Expression of DmGW182-delCterm-delM2-dsRNAresDepositorInsertDmGW182-delCterm-delM2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J
Plasmid#146691PurposeInsect Expression of DmHPat-del57-499-dsRNAresDepositorInsertDmHPat-del57-499-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA
Plasmid#194249PurposeExpresses 3 non-targeting miRNAsDepositorInsertmiRNegative
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-EGFL7-COMP5AP-AviTag-9xHis
Plasmid#157266PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertEGFL7 (EGFL7 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CBA::EGFP-P2A-tC3-FLAG
Plasmid#129477PurposeAAV2 transfer plasmid for truncated C3 tagged with FLAG with EGFP under control of the CBA promoterDepositorInsertFLAG-tagged (C-terminus) truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-3xL-Amut-dsRNAres_P
Plasmid#147160PurposeInsect Expression of DmNot1-3xL-Amut-dsRNAresDepositorInsertDmNot1-3xL-Amut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1147-1717-3xL-Amut_P
Plasmid#147161PurposeInsect Expression of DmNot1_1147-1717-3xL-AmutDepositorInsertDmNot1_1147-1717-3xL-Amut (Not1 Fly)
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL E167D (siRNA resistant)
Plasmid#191013PurposeExpresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationE167DPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-7-SV40 polyA
Plasmid#190871PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode7
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-8-SV40 polyA
Plasmid#190872PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode8
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-6-SV40 polyA
Plasmid#190870PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode6
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-eGFP-P2A-CRY2-VPR
Plasmid#192610PurposeLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.DepositorInserteGFP-P2A-CRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagseGFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_1-671-del286-316_AI
Plasmid#148944PurposeMammalian Expression of HsGIGYF1_1-671-del286-316DepositorInsertHsGIGYF1_1-671-del286-316 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI
Plasmid#148949PurposeMammalian Expression of HsGIGYF2_1-718-del280-310DepositorInsertHsGIGYF2_1-718-del280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W
Plasmid#147888PurposeMammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_W
Plasmid#147890PurposeMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)
Plasmid#191174PurposeMammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.DepositorInsertCMV-TO:mCherry:s70587:EGFP
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)
Plasmid#191150PurposeExpression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L153:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)
Plasmid#191152PurposeExpression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L36:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only