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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1

Plasmid

#206260

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 1

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 3

Plasmid

#206262

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 3

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4

Plasmid

#206263

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 4

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA6-LRRC55-V5-Flag-His

Plasmid

#127819

Purpose

Expression of human LRRC55 in mammalian cells

Depositor

Jiusheng Yan

Article

Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

Insert

LRRC55 (LRRC55 Human)

Tags

V5, FLAG, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109AsAmp30E

Plasmid

#123144

Purpose

Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP2-SH2

Plasmid

#46493

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP2

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-131

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP1FBAR(516a.a.)-EGFP

Plasmid

#168505

Purpose

Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1-FBAR (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-516

Promoter

CAG

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Article

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Tags

dsRed-monomer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJR2956

Plasmid

#122533

Purpose

Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

PBen

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGATOR

Plasmid

#64102

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-AS

Plasmid

#16448

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 antisense (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMpGWB406

Plasmid

#68671

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98N-pBabe-puro

Plasmid

#19236

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Asparagin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJTX-GL3

Plasmid

#11981

Purpose

Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
RBM23_2xRRM_pGEX

Plasmid

#135118

Purpose

Recombinant expression of RBM23 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM23 (RBM23 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB431

Plasmid

#68696

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GammaPCDH-A3-GFP/pEGFP-N1

Plasmid

#197343

Purpose

Plasmid expressing an antigen targeting Pan-Gamma-protocadherin-Constant

Depositor

Joshua Weiner

Article

Lobas et al J Neurochem. 2012 Mar;120(6):913-27. doi

Insert

pcdh-gamma-A3

Tags

GFP (GFP observed)

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN41A-T38S38

Plasmid

#49524

Purpose

Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Promoter

Ptb38

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN12A-P750

Plasmid

#49525

Purpose

Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

Plasmid

#97010

Purpose

Rosa26 targeting vector for FLP-responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PIWI

Plasmid

#11591

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Mutation

contain only aa482-869

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DEP-GFP

Plasmid

#109043

Purpose

Expression of the Dishevelled DEP domain

Depositor

Mariann Bienz

Article

Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

Insert

dishevelled segment polarity protein 2 (DVL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Dvl2 DEP domain

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pMCL-HA-MAPKK2-KW71[delta(48-55)/S222D/S226D]

Plasmid

#40821

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 6441 - 6480 of 20363 results

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We narrowed to 20,363 results for: ren