We narrowed to 18,681 results for: tec

1. pTK296

   Plasmid

   #59565

   Purpose

   SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(14) 2xK-turn Kozak EGFP-PEST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Neq2X

   Plasmid

   #182365

   Purpose

   Expresses Neq2X DNA polymerase

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

   Insert

   Neq2X
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YEp181-CUP1-His-Smt3

   Plasmid

   #99536

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A_dCas9-1x2

   Plasmid

   #63596

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGNW6

   Plasmid

   #122093

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PlayBack-CMV-Kpn1

   Plasmid

   #203302

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLH-sgRNA1-boxB-MS2-PP7

   Plasmid

   #75397

   Purpose

   sgRNA1-boxB-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. apt-ABE8e

    Plasmid

    #219937

    Purpose

    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Com_93-aa_TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. IVT-MCP-MMLV-RT

    Plasmid

    #207457

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gata4-GFP donor

    Plasmid

    #113115

    Purpose

    Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Gata4 donor region including homology arms and eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Use

    Tags

    G protein and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

14. BiP-mGFP P495L

    Plasmid

    #62232

    Purpose

    Mutant of hamster BiP-mGFP unable to bind clients.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 TCF12

    Plasmid

    #67113

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF12 (TCF12 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a.Tat(48-57)-I-SceI

    Plasmid

    #165041

    Purpose

    Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    I-SceI
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-O-FUW-Olig2

    Plasmid

    #45844

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCHERRY10

    Plasmid

    #24664

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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