We narrowed to 9,959 results for: Gnas
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Plasmid#212903PurposeEncodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD002
Plasmid#212899PurposeEncodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCRH1 (CRH1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD007
Plasmid#212904PurposeEncodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertOST1 (OST1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD004
Plasmid#212901PurposeEncodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD009
Plasmid#212906PurposeEncodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSRL1 (SRL1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-EGFR
Plasmid#208280PurposeExpresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-EGFR
UseLentiviralMutationH225N in mCherry- please see depositor commentPromoterpCMVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210736PurposeCoding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Merry sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210735PurposeCoding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Gollum sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-PTS1
Plasmid#209398PurposeTransiently expressing TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-PT2A
Plasmid#202138PurposeLevel 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructsDepositorInsertDual 2A peptide
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAAV-CAG-miniSOG-C-Far
Plasmid#193914PurposeControl plasmid that expresses miniSOG targeted to the cell membrane in mammalian cellsDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianPromoterCAGAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xHAtag-SP-CLSTN2
Plasmid#191694PurposeFor expression coding sequencing of CLSTN2DepositorInsertsignal peptide, 3xHAtag and full length coding sequence of CLSTN2
ExpressionMammalianMutationWTAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SP-3xHAtag-PTPRO
Plasmid#191695PurposeFor expression coding sequencing of PTPRODepositorInsertsignal peptide, full length coding sequence of PTPRO and 3xHAtag
ExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
nPPARg429-gs10-NLS-VPR
Plasmid#188266PurposePlasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnPPARg429 (PPARG Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
iLID-mRuby3-iTrkA
Plasmid#136510PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)DepositorAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
iLID-mRuby3-iTrkB
Plasmid#136511PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)DepositorInsertiLID mRuby3 iTrkB
ExpressionMammalianPromoterCMVAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-MTS-GFP11-Ura
Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
ExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mSpata33-PA
Plasmid#179129PurposeExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2S31A
Plasmid#174332Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsmCherry tagExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12-MpER-Targ
Plasmid#136094PurposeCDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'endDepositorInsertER targeting peptide from predicted chitinase Mapoly0069s0092
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3.1-NKD2S31A
Plasmid#174331Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsMycHisExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASP-3G124-HilA
Plasmid#153324PurposeShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vectorDepositorInsert3G124 DARPin but no expression
UseSalmonella expressionAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
Tags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
Tags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE NLS
Plasmid#82465PurposeExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminusDepositorInsertCapping Enzyme (Rngtt Mouse)
TagsMycExpressionMammalianMutationdeleted amino acids Nuclear Localization SignalPromoterCMVAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE
Plasmid#82463PurposeExpression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cellsDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT1
Plasmid#67116PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_PTS1
Plasmid#65699PurposeExpresses YNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsYNLExpressionMammalianPromoterCMVAvailable SinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_PTS1
Plasmid#65700PurposeExpresses CNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsCNLExpressionMammalianPromoterCMVAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_PTS1
Plasmid#65701PurposeExpresses ONL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsONLExpressionMammalianPromoterCMVAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 EE Jip1b
Plasmid#52127Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPL13
Plasmid#31344DepositorInsertmitochondrial ribosomal protein L13 mature form (MRPL13 Human)
TagsHisExpressionBacterialMutationMature form after removal of the predicted mitoch…Available SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only