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Showing: 3241 - 3260 of 4130 results

  2. pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

    Plasmid
    #236278
    Purpose
    Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.
    Depositor
    Arnab Mukherjee
    Article
    Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.
    Insert
    human aquaporin 1 (AQP1 Human)
    Use
    Lentiviral
    Tags
    Flag and fkbp12
    Expression
    Mammalian
    Promoter
    CMVtight
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236278-plasmid-map-sequence-id-474326

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  5. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

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  6. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  7. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  8. pCA528 CHMP2A 152-222

    Plasmid
    #104594
    Purpose
    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP2A residues 152-222 (CHMP2A Human)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeli…
    Promoter
    T7
    Available Since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    104594-plasmid-map-sequence-id-245144

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  9. pCHMP2A-mut-NS3-Green

    Plasmid
    #223529
    Purpose
    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing a mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP2A (CHMP2A Human)
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223529-plasmid-map-sequence-id-443350

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  10. GPBA-DuET

    Plasmid
    #213255
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPBA (GPBAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213255-plasmid-map-sequence-id-430805

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  15. qTAG-N-Solo-mScarlet-ACTB

    Plasmid
    #207750
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207750-plasmid-map-sequence-id-414814

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  16. qTAG-N-Solo-mNeon-ACTB

    Plasmid
    #207749
    Purpose
    Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207749-plasmid-map-sequence-id-414795

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  20. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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Showing: 3241 - 3260 of 4130 results