4,178 results

1. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p212 pCMV-EGFP/RFP

   Plasmid

   #8389

   Purpose

   Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKH3-human RSK1

   Plasmid

   #13841

   Depositor

   John Blenis

   Article

   Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

   Insert

   RSK1 (RPS6KA1 Human)
   

   Tags

   3x HA

   Expression

   Mammalian

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage-ubc-nls-ha-tdPCP-gfp

   Plasmid

   #40650

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdPCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-IRES-CFP II (pMIC II)

   Plasmid

   #52109

   Purpose

   a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-ECFP

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2xFLAGhYAP1-S127A

   Plasmid

   #17790

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein mutant S127A (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Serine 127 to Alanine

   Available Since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN4-EF1a-ETV2-IRES-Puro

   Plasmid

   #61061

   Purpose

   lentiviral vector for ETV2

   Depositor

   Igor Slukvin

   Article

   Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Lentiviral

   Promoter

   EF1alpha

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-GoldyTALEN

   Plasmid

   #38143

   Purpose

   destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

   Depositor

   Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Tags

   AcV5 and FokI homodimer

   Expression

   Mammalian

   Mutation

   Truncate at N and C terminous

   Promoter

   miniCaggs

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV-MokolaG

    Plasmid

    #15811

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Mokola virus glycoprotein (G) gene
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. eIF2a 3

    Plasmid

    #21809

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Expression

    Mammalian

    Mutation

    S52D

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAmCherry-b-actin

    Plasmid

    #31949

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Tags

    b-actin

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    SV40 large T
    

    Expression

    Mammalian

    Available Since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-ReaChR-citrine

    Plasmid

    #50954

    Purpose

    ReaChR-citrine in AAV2 vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    AAV

    Tags

    citrine

    Promoter

    truncated human Synapsin promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe EGFR(T790M)

    Plasmid

    #32070

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-Myc-ASC

    Plasmid

    #73952

    Purpose

    Express c-Myc-tagged human ASC in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

    Insert

    PYD and CARD domain containing, PYCARD (PYCARD Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-PuroR

    Plasmid

    #71667

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28c-F30-2xdBroccoli

    Plasmid

    #66843

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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