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Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-AktAR2
Plasmid#184048PurposeCytosol-targeted ExRai-AktAR2.DepositorInsertCyto-ExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG058
Plasmid#214261PurposePaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)DepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
UseTags6xHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
UseTagsExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pGEMHE
Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorInsertOtopetrin 2 (Otop2 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarca4
Plasmid#122146PurposeFor making Smarca4 RNADepositorInsertSmarca4 (Smarca4 Mouse)
UseFor in vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-AREL1
Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorInsertKIAA0317 (AREL1 )
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertBetacellulin (BTC Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJA1
Plasmid#177655PurposeRecombinant protein synthesis of human DNAJA1DepositorInsertDNAJA1 (DNAJA1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
UseTags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
rTetR-NS3-VP64-p65_IRES_mCherry
Plasmid#112628PurposeEncodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.DepositorInsertrTetR-NS3-VP64-p65
UseTagsAU1 (N term on NS3), HA (C term on NS3), and T7 (…ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N24_switch
Plasmid#132725PurposeT7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N24_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_switch
Plasmid#132722PurposeT7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N20_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_trigger
Plasmid#132726PurposeT7 RNAP-driven expression of 3WJ repressor index 20 trigger RNADepositorInsert3WJ_N20_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-3xFlag-IFIT5
Plasmid#53561Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags3xFLAG, 6xHIS, and TEV siteExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
(294) pcDNA3.1-myc-OGA(1-400)
Plasmid#168095PurposeMyc tagged truncated human OGA catalytic domain of residues 1-400DepositorInsertmyc-OGA(1-400)
UseTagsmycExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK4
Plasmid#122675PurposeExpresses mouse PCSK4 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
RC(WW)-LgBit
Plasmid#208931PurposeRecognition domain for in vitro MAPK-activity sensorDepositorInsertWW domain of PIN1 (PIN1 Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His-VCre
Plasmid#123131PurposeBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.DepositorInsertVCre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA
Plasmid#168101PurposeGFP and Flag tagged Nup62 with EPEA nanobody tagDepositorInsertGFP-Flag-Nup62-EPEA
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-drebrin
Plasmid#40362DepositorInsertdrebrin E2 (DBN1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT-SuperFi-Cas9
Plasmid#183630PurposeExpression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coliDepositorInsertSuperFi-Cas9
UseCRISPRTags6xHis-MBPExpressionBacterialMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …PromoterT7Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGP-RSET-iGABASnFR2n-6xHis
Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
UseTags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only