We narrowed to 10,761 results for: T7
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Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
ExpressionBacterialPromoterPT7Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
TagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialPromoterT7Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-1
Plasmid#136385PurposeExpresses DANCER-1 dynamic proteinDepositorInsertDANCER-1
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-2
Plasmid#136386PurposeExpresses DANCER-2 dynamic proteinDepositorInsertDANCER-2
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39LPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-S
Plasmid#136388PurposeExpresses NERD-S static proteinDepositorInsertNERD-S
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39I-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F/V39L)
Plasmid#136610PurposeExpresses GB1 WT double mutant (A34F/V39L)DepositorInsertGB1 WT double mutant (A34F/V39L)
Tags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34F - V39LPromoterT7 promoterAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNusGNTD
Plasmid#113123PurposeExpresses N-terminus of E. coli NusGDepositorInsertNusG
TagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 120-181PromoterT7Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06_ZIKV NS4A
Plasmid#145356PurposeCell-free expression vector for Zika virus NS4A protein with C-term mCherryDepositorInsertZIKV NS4A
TagsmCherry and 8xHisExpressionBacterialPromoterT7Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
Tags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
TagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-ammA(∆50-56)
Plasmid#134684PurposeExpresses His6-AmmA(∆50-56) in E. ColiDepositorInsertammA(del50-56)
TagsHis6ExpressionBacterialMutationdeletion of amino acids 50-56PromoterT7Available SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz106_CsoS2_MR
Plasmid#140843PurposeHis-tagged CsoS2 Middle Region (MR), pET-14b backboneDepositorInsertCsoS2 Middle Region (MR)
TagsHexahistidine tagExpressionBacterialMutationonly amino acids 257-603PromoterT7Available SinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
TagsHexahistidine tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialPromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet139
Plasmid#121913PurposeCharge-Introduced NpuDnaB mini-inteinDepositorInsertCI-NpuDnaBΔ290 intein
TagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet140
Plasmid#121914PurposeOrthogonal NpuDnaB mini-intein inteinDepositorInsertOth-NpuDnaBΔ290 intein
TagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-S15
Plasmid#127821PurposeProduction of recombinant human ribosomal protein S15DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S10
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationW22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_WTTS
Plasmid#91227Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank1a_pJet1.2
Plasmid#121978Purposefor generating kank1a riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
Tags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7R8
Plasmid#111041PurposePlasmid expresses the talin2 rod domains R7 and R8DepositorAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7
Plasmid#111039PurposePlasmid expresses the talin2 rod domain R7 a 5 helix bundle.DepositorAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A
Plasmid#109031PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
TagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR161Q
Plasmid#92413PurposehQPRTase harboring R161Q mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR161QPromoterT7Available SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC(Q120L)
Plasmid#99702PurposeBacterial expression of RagA(Q66L)-RagC(Q120L)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120L and Q66LPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only