We narrowed to 8,806 results for: CAG

1. lentiCRISPR v2-sgCYFIP1-2

   Plasmid

   #210138

   Purpose

   knock out CYFIP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

   Plasmid

   #204620

   Purpose

   Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9_ANKRD1_sgRNA2

   Plasmid

   #186669

   Purpose

   Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_BRD003 LIPT2-1

   Plasmid

   #184482

   Purpose

   Lentivirus gRNA targeting human LIPT2 gene

   Depositor

   Peter Tsvetkov, Todd Golub

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   LIPT2 (LIPT2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT22UbGfpnE4PCh(#301)

   Plasmid

   #184066

   Purpose

   cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

   Insert

   eGFP-nls-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-MmCavin4

   Plasmid

   #170528

   Purpose

   Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Cavin4 (Cavin4 Mouse)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-MmCav1

   Plasmid

   #170527

   Purpose

   Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Caveolin1 (Cav1 Mouse)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CBSH3- 3m shRNA3

    Plasmid

    #160213

    Purpose

    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CBSH3- 3m shRNA2

    Plasmid

    #160211

    Purpose

    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CBSH3- 3m shRNA1

    Plasmid

    #160209

    Purpose

    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MARK1 gRNA (BRDN0001146169)

    Plasmid

    #77795

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MARK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MARK1 (MARK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. IRAK2 gRNA (BRDN0001146650)

    Plasmid

    #77729

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IRAK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IRAK2 (IRAK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. IRAK2 gRNA (BRDN0001146975)

    Plasmid

    #77730

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IRAK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IRAK2 (IRAK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDC42BPG gRNA (BRDN0001144777)

    Plasmid

    #77528

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDC42BPG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDC42BPG (CDC42BPG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MYLK gRNA (BRDN0001147172)

    Plasmid

    #77021

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MYLK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MYLK (MYLK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HIPK1 gRNA (BRDN0001144888)

    Plasmid

    #76995

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HIPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HIPK1 (HIPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DGKA gRNA (BRDN0001144932)

    Plasmid

    #76608

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKA (DGKA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ERBB4 gRNA (BRDN0001149467)

    Plasmid

    #76196

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERBB4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CDK11A gRNA (BRDN0001147530)

    Plasmid

    #75892

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK11A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK11A (CDK11A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. scAAV-Cre

    Plasmid

    #177933

    Purpose

    Expresses Cre-recombinase and can be packaged into AAV particles.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Insert

    CAG-Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

    Plasmid

    #210017

    Purpose

    episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for high-quality integration-free naïve reset; best for rodents and primates (including human).

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sox2A61V is a point mutant of Sox2 that enhances …

    Promoter

    CAG

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCXLE-mCherry-t2a-SOX2-p2a-KLF4

    Plasmid

    #193296

    Purpose

    episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV uN2C GFP (Ctl)

    Plasmid

    #224355

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intyron (CAG)

    Available since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid

    #212967

    Purpose

    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    

    Use

    CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, T7 and U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge