We narrowed to 11,284 results for: Vars;

1. pEMS2232

   Plasmid

   #158401

   Purpose

   High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple316 (NR2E1 Human)
   

   Use

   Unspecified

   Promoter

   Ple316

   Available Since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 6xHis-MBP-BrCas12b

   Plasmid

   #182276

   Purpose

   Expresses recombinant BrCas12b

   Depositor

   Piyush Jain

   Article

   Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, T7, Thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-RON-V5/HIS

   Plasmid

   #201983

   Purpose

   expression of human RON receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RON receptor tyrosine kinase (MST1R Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR D837A

   Plasmid

   #11014

   Purpose

   Retroviral construct for expressing human EGFR with D837A mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D837A (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D837A

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pUG-natNT2

   Plasmid

   #110922

   Purpose

   expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   nat
   

   Expression

   Bacterial and Yeast

   Promoter

   AgTEF1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW_0065

   Plasmid

   #102817

   Purpose

   pVec8 containing Sr22 gene

   Depositor

   Brande Wulff

   Article

   Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

   Insert

   wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)
   

   Expression

   Plant

   Promoter

   Native

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW_0059

   Plasmid

   #102819

   Purpose

   pAGM4723 containing Sr35 gene

   Depositor

   Brande Wulff

   Article

   Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

   Insert

   wheat stem rust resistance gene Sr35 (NEWENTRY Synthetic)
   

   Use

   Synthetic Biology

   Promoter

   Native

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-N-B.1.1.7

   Plasmid

   #177958

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
   

   Expression

   Mammalian

   Mutation

   D3L,R203K,G204R,S235F

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-A11(89-196)

   Plasmid

   #196215

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-A11(2-68)-Strep

    Plasmid

    #196216

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GB1-A11(2-52)-Strep

    Plasmid

    #196213

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDual-eGFP(Y203)

    Plasmid

    #63559

    Purpose

    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Y203)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed threonine at position 203 with respect to…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-EFS-DjCas13d-HA-mCherry-pA

    Plasmid

    #233030

    Purpose

    To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW_0141

    Plasmid

    #102820

    Purpose

    pVec8 containing Sr45 gene

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)
    

    Expression

    Plant

    Promoter

    Sr33

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-FLEX-ArgiNLS-AausFP1

    Plasmid

    #220602

    Purpose

    Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist-SARS-CoV-2 Δ18 P.1

    Plasmid

    #173476

    Purpose

    Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

    Plasmid

    #194378

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Tags

    Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe EGFR(L861Q)

    Plasmid

    #32068

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L861Q

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

21. HA-mRIN2 dRA

    Plasmid

    #108941

    Purpose

    Mammalian expression of mRIN2 dRA

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RA domain (aa 751-836)

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. HA-mRIN2 dRHVPS9

    Plasmid

    #108942

    Purpose

    Mammalian expression of mRIN2 dRHVPS9

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RHVPS9 domain (aa 455-738)

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1-RYK-V5/HIS

    Plasmid

    #205610

    Purpose

    Expression of human RYK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RYK receptor tyrosine kinase (RYK Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCPH2A.X_S139D-GS3

    Plasmid

    #204748

    Purpose

    Expression of Cas9 and human H2AX with the S139D mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139D (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the S139D mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. mTFP1-Caveolin-C-10

    Plasmid

    #55468

    Purpose

    Localization: Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    NM_001753 (CAV1 Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

    Plasmid

    #221224

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCK340

    Plasmid

    #192639

    Purpose

    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpG has se…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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