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Showing: 8201 - 8250 of 9723 results

  3. pDD2601

    Plasmid
    #175085
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Dirk Dittmer
    Article
    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Expression
    Mammalian
    Mutation
    S47A, S65A, T75A
    Available Since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    175085-plasmid-map-sequence-id-345858

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  4. iDianiSnFR

    Plasmid
    #177741
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iDianiSnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177741-plasmid-map-sequence-id-348683

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  5. iCytSnFR

    Plasmid
    #177738
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCytSnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177738-plasmid-map-sequence-id-348679

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  6. iCyt_BrEt_SnFR

    Plasmid
    #177740
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_BrEt_SnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177740-plasmid-map-sequence-id-349387

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  7. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_F_SnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177739-plasmid-map-sequence-id-348682

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  9. pCCM’ CbbQ-

    Plasmid
    #162711
    Purpose
    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    Inactivation of CbbQ subunit of CbbOQ rubisco act…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162711-plasmid-map-sequence-id-324197

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  10. Tac(5A)-GFP

    Plasmid
    #162495
    Purpose
    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162495-plasmid-map-sequence-id-323475

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  11. GFP-Tac(5A)

    Plasmid
    #162493
    Purpose
    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162493-plasmid-map-sequence-id-323471

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  12. pMinDis.X513-iNicSnFR3b-(V7)-ER

    Plasmid
    #125123
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(V7)-ER
    Tags
    Fusion protein -Endoplasmic retention motif
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125123-plasmid-map-sequence-id-245436

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  17. PAK4-FL-3'UTR-MT2

    Plasmid
    #113093
    Purpose
    Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated
    Depositor
    James Donahue
    Article
    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840
    Insert
    p21 Activated kinase 4 (PAK4 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Second predicted miR-199a-3p binding site mutated…
    Promoter
    PGK
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113093-plasmid-map-sequence-id-216990

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  19. pCIG1_UL136_26kDa_Myc

    Plasmid
    #74955
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)
    Expression
    Mammalian
    Mutation
    delta (1-62) M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74955-plasmid-map-sequence-id-141778

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  20. pCIG1_UL136_33kda_Myc

    Plasmid
    #74957
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Myc tag (EQKLISEEDL)
    Expression
    Mammalian
    Mutation
    M63A M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74957-plasmid-map-sequence-id-141779

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  35. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

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  43. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid
    #234951
    Purpose
    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    234951-plasmid-map-sequence-id-467430

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  44. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  45. pAAV-UBC-XRI-HA

    Plasmid
    #178056
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-HA
    Use
    AAV
    Tags
    HA-MBP
    Expression
    Mammalian
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178056-plasmid-map-sequence-id-349205

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  47. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  48. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid
    #234952
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234952-plasmid-map-sequence-id-467733

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  49. pLJM1-PM-RA-BlastR

    Plasmid
    #211708
    Purpose
    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    PM-targetted RA (Lck Synthetic)
    Use
    Lentiviral
    Tags
    RA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211708-plasmid-map-sequence-id-419204

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Showing: 8201 - 8250 of 9723 results