We narrowed to 27,291 results for: Tat

1. pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

   Plasmid

   #62576

   Purpose

   Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   RASA1 (RASA1 Human)
   

   Use

   Luciferase

   Tags

   None

   Expression

   Mutation

   miR-206 binding site mutated (ACATTCCA --> AAC…

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-PDE4D2cat-ICUE4

   Plasmid

   #181847

   Purpose

   ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
   

   Use

   Tags

   ECFP, PDE4D2(86-418), and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-Flag-SPRTN-E112A

   Plasmid

   #110215

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E112A, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4-eIF3B MBE3 mutant

   Plasmid

   #133310

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE3 mutant (EIF3B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   ccacgtgacc changed to cAaAAAAacc

   Promoter

   eIF3B

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4-eIF3B MBE2 mutant

   Plasmid

   #133309

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE2 mutant (EIF3B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   gccacatgcacc changed to gcAaAaAAcacc

   Promoter

   eIF3B

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

   Plasmid

   #183080

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   -124C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

   Plasmid

   #183081

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   -146C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 BA.2.75 Spike

   Plasmid

   #190012

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.2.75 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the BA.2 mutations (T19I, LPPA24S, G142D…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Sept. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti-EML4-ALK variant 3a I1171N

   Plasmid

   #183832

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid

    #183079

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Tags

    Expression

    Mutation

    Wildtype promoter, Exon 1 C7C (C21>T21) silent…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid

    #124257

    Purpose

    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Arginine 132 is mutated to Histidine. This mutati…

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EML4-ALK variant 3a G1202R

    Plasmid

    #183830

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    G1202R

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid

    #183831

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    L1196M/G1202R

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FR52re

    Plasmid

    #45489

    Depositor

    David Baker

    Insert

    FR52re
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    redesign of Di-I_5_FR28

    Promoter

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PL2x2_5

    Plasmid

    #45491

    Depositor

    David Baker

    Insert

    PL2x2_5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed E30Y of Di-IV_5_PL2x2_6

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

17. FR55

    Plasmid

    #45490

    Depositor

    David Baker

    Insert

    FR55
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed F14L and added Gly at N-terminus of Di-I_…

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

18. control (pGS701)

    Plasmid

    #160037

    Purpose

    pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCGLH-Flna

    Plasmid

    #194980

    Purpose

    knock down FLNA

    Depositor

    Angelique Bordey

    Insert

    FLNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN_empty

    Plasmid

    #140404

    Purpose

    Empty backbone with nourseothricin selection in plants

    Depositor

    Mathias Pribil

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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