We narrowed to 18,889 results for: Tec

1. pCfB2225

   Plasmid

   #67553

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSGV Hu Acceptor PGK-NGFR

   Plasmid

   #64270

   Purpose

   Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30-T7-His-sfGFP

   Plasmid

   #180755

   Purpose

   pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Tags

   strep tag

   Expression

   Bacterial

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKD227

   Plasmid

   #90954

   Purpose

   Expresses S. baltica TtrS under the Ptac promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Insert

   Tetrathionate sensor kinase
   

   Expression

   Bacterial

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB6576

   Plasmid

   #106134

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 TFEC

   Plasmid

   #67060

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFEC (TFEC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDRFLIP38

   Plasmid

   #65516

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK295

   Plasmid

   #59564

   Purpose

   SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(14) 2xK-turn Kozak EGFP
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB5791

   Plasmid

   #106126

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265_OoA3GX2

    Plasmid

    #225147

    Purpose

    Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    OoA3GX2-zCas9(D10A)-UGI
    

    Expression

    Plant

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xNLS-pMJ915v2

    Plasmid

    #88916

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

    Plasmid

    #48296

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Insect

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTnT-Flp-3xFLAG

    Plasmid

    #109167

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSD2-FLAG

    Plasmid

    #24097

    Depositor

    Moses Chao

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

    Insert

    HSD2 (HSD11B2 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW842_pCAG-pExpr-FP-DECODER

    Plasmid

    #87551

    Purpose

    Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP, EGFP, iRFP720, mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

    Plasmid

    #55217

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Available Since

    July 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

    Plasmid

    #50022

    Purpose

    expresses Membrane tagged G-CaMP3 in cells expressing Cre

    Depositor

    David Anderson

    Article

    Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

    Insert

    G-CaMP3
    

    Use

    AAV

    Tags

    MARCKS sequence (MGCCFSKT)

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only