We narrowed to 9,597 results for: EPO

1. CIP2Aprom285bp-pGL4.10Luc

   Plasmid

   #60873

   Purpose

   Luc reporter driven by 285 bp of the CIP2A promoter

   Depositor

   Jukka Westermarck

   Article

   Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

   Insert

   285 bp promoter fragment of Cancerous Inhibitor of PP2A2 (CIP2A Human)
   

   Use

   Luciferase; Promoterless

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pEMS1210

   Plasmid

   #29107

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple238 (TRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMiR-RECK-3'UTR-miR-7-mut

   Plasmid

   #53692

   Purpose

   Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

   Depositor

   Edward Chan

   Article

   Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

   Insert

   RECK-3'UTR (RECK Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   nucleotide position 171-174 are mutated to GAAG

   Promoter

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

   Plasmid

   #226115

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-107 Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

   Plasmid

   #226109

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-92a Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

   Plasmid

   #226110

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-378a Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

   Plasmid

   #226112

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-33b Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_ER-RE_MLPmin_BC1064-luc2

   Plasmid

   #227110

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1064

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1064 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs-uORFx6-mNgn2x3HA-P2A-mRuby2

   Plasmid

   #233168

   Purpose

   Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
   

   Use

   Retroviral

   Tags

   x3HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-uORFwx1-Isl1-P2A-mRuby2

    Plasmid

    #233170

    Purpose

    Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXs-uORFx1-Isl1-P2A-mRuby2

    Plasmid

    #233171

    Purpose

    Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs-uORFx2-Isl1-P2A-mRuby2

    Plasmid

    #233172

    Purpose

    Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-uORFx3-Isl1-P2A-mRuby2

    Plasmid

    #233173

    Purpose

    Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-uORFx6-Isl1-P2A-mRuby2

    Plasmid

    #233174

    Purpose

    Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-uORFwx1-mLhx3-P2A-mRuby2

    Plasmid

    #233176

    Purpose

    Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-uORFx1-mLhx3-P2A-mRuby2

    Plasmid

    #233177

    Purpose

    Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-uORFx2-mLhx3-P2A-mRuby2

    Plasmid

    #233178

    Purpose

    Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-uORFx3-mLhx3-P2A-mRuby2

    Plasmid

    #233179

    Purpose

    Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-uORFx6-mLhx3-P2A-mRuby2

    Plasmid

    #233180

    Purpose

    Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233164

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs-uORFx1-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233165

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233166

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233167

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXJ-Nluc-_(PLRSKK)-CA1-19

    Plasmid

    #232657

    Purpose

    Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CGA (CGA Human)
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid

    #229810

    Purpose

    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-AmCyan-stop-lox
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225588

    Purpose

    Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2(S54L+T127A)-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225586

    Purpose

    Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    F8.2(S54L+T127A)-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV_AR-RE_MLPmin_BC1013-luc2

    Plasmid

    #227107

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1013

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HIV-1-GFP ΔEnv CA Q63/67A

    Plasmid

    #217439

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    CA Q63A,Q67A

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HIV-1-GFP ΔEnv CA Q67H/N74D

    Plasmid

    #217441

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    CA Q67H,N74D

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid

    #201539

    Purpose

    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)

    Depositor

    Joshua Leonard

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Inserts

    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_GR-RE_MLPmin_BC1093-luc2

    Plasmid

    #227113

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pmiRGLO-Zc3h12a 3’UTR

    Plasmid

    #222662

    Purpose

    Luciferase reporter vector containing mouse Zc3h12a 3'UTR

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a 3'UTR (Zc3h12a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

    Plasmid

    #224788

    Purpose

    SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crCoV20, crCoV21, crCoV24
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

    Plasmid

    #226101

    Purpose

    HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SIFI degron helices 1 & 2 deleted (Δ61-112)

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

    Plasmid

    #226100

    Purpose

    HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only N-HRI (aa 1-138)

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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39. pLV-GG-hUbC-EBPF2-gRNA-FAM13A

    Plasmid

    #185550

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    FAM13A gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPRKCZ-C20-EGFP (C-PRKCZ-C20)

    Plasmid

    #217761

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

    Plasmid

    #217763

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

    Plasmid

    #217762

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Linker GGSSGGGA plus C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pJT314 AAV-Rapamycin Inducible-NZ-Citrine

    Plasmid

    #202063

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NZ-Citrine
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJT365 AAV-Rapamycin Inducible-S3H

    Plasmid

    #202066

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-S3H
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJT366 AAV-Rapamycin Inducible QNZF

    Plasmid

    #202067

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible QNZF
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJT313 AAV-Rapamycin Inducible-ZF-Citrine

    Plasmid

    #202062

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-ZF-Citrine
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. p2705-AGER-eGFP

    Plasmid

    #216474

    Purpose

    donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pHAND1-T2A-dTom-PGK-Neo

    Plasmid

    #209838

    Purpose

    Tomato reporter plasmid targeting the human HAND1 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

    Insert

    HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pXPR_068

    Plasmid

    #164555

    Purpose

    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge