We narrowed to 10,893 results for: ENA;

1. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R, R883K, R887A

   Promoter

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Delta DED FLIP-L

   Plasmid

   #13790

   Depositor

   Guy Salvesen

   Article

   Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

   Insert

   DELTA DED FLIP LONG (CFLAR Human)
   

   Use

   Tags

   8 x His tag

   Expression

   Bacterial

   Mutation

   Delta DED removes first 186 residues

   Promoter

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   PBen

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-Blast SHMT1

   Plasmid

   #106304

   Purpose

   Express SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 1 (SHMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-mCherry-N

   Plasmid

   #210271

   Purpose

   N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEAST-BenR

   Plasmid

   #114597

   Purpose

   "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   OR2-OR1-Pr

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_791

   Plasmid

   #216079

   Purpose

   Minimal plasmid; contains only ori and ampR, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Destination

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA295

   Plasmid

   #215986

   Purpose

   Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; SV40NLS_v1.19
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AA014

    Plasmid

    #216057

    Purpose

    Fragmid fragment: (2A Selection) Puromycin resistance

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    P2A_v1.1; PuroR_v1.1; Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AA163

    Plasmid

    #216061

    Purpose

    Fragmid fragment: (2A Selection) Stop only

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AA065

    Plasmid

    #215965

    Purpose

    Fragmid fragment: (Pol 2 promoter) human EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EF1a_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPKm-202

    Plasmid

    #90492

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    CMVmin Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEY22

    Plasmid

    #191028

    Purpose

    lim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    lim-4 (lim-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEY17

    Plasmid

    #191023

    Purpose

    flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-18 CELE_Y48D7A.2 (flp-18 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEY16

    Plasmid

    #191022

    Purpose

    flp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-6 (flp-6 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEY18

    Plasmid

    #191024

    Purpose

    flp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-19 (flp-19 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEY19

    Plasmid

    #191025

    Purpose

    flp-26 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-26 (flp-26 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEY21

    Plasmid

    #191027

    Purpose

    ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    ggr-3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEY23

    Plasmid

    #191029

    Purpose

    pdfr-1(2-1k) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    pdfr-1(2-1k) (pdfr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge