We narrowed to 7,952 results for: Lif

Showing: 3301 - 3320 of 7952 results

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pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218879

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Has Service

AAV1 and AAV5

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188765

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GC1 pComb3xss anti-Gephyrin nanobody

Plasmid

#134720

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-AausFP1

Plasmid

#191096

Purpose

To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-AausFP1

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

Optimized to Human codon usage

Promoter

CMV enhancer and CAG

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut2 [N29/29R]

Plasmid

#114501

Purpose

Mammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-WAVE1/SCAR [K91/36R]

Plasmid

#128629

Purpose

Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4404

Plasmid

#115465

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4404 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-cmlc2-eGFP

Plasmid

#117770

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3755 : HDR-HsIRE1a-10xGS-HaloTag

Plasmid

#185677

Purpose

HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 homology arms (ERN1 Human, Synthetic)

Use

CRISPR

Tags

HaloTag

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-SNAPf-CCR5TC

Plasmid

#135536

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

SNAPf

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mAtp6v1a-1

Plasmid

#198477

Purpose

lentiviral stable expression of mAtp6v1a gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse ATPv1a (Atp6v1a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mAtp6v1a-2

Plasmid

#198478

Purpose

lentiviral stable expression of mAtp6v1a gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse ATPv1a (Atp6v1a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-ATG

Plasmid

#153429

Purpose

Expresses a gRNA that overlaps the startcodon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-TRPS1-P2A-mCherry

Plasmid

#203851

Purpose

Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

TRPS1 (TRPS1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-TagBFP

Plasmid

#117777

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#234754

Purpose

Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TRPML1(PHD)

Plasmid

#221752

Purpose

Stable expression of GFP-TRPML1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

TRPML1 (MCOLN1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TRPML1(PHD) (amino acids 1–69)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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