163,606 results

1. pCAT203

   Plasmid

   #134880

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-FB-dCas9-puro

   Plasmid

   #100547

   Purpose

   Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Inserts

   N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
   puromycin
   

   Use

   CRISPR

   Tags

   FLAG, Biotin-acceptor-site (Avitag)

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGal4BD-CRY2PHR

   Plasmid

   #28244

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   cry2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSM.wrmScarlet1-10::SL2::GFP1-10

   Plasmid

   #234318

   Purpose

   For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

   Insert

   wrmScarlet1-10::SL2::GFP1-10
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCG-TPMV Hd32

   Plasmid

   #163963

   Purpose

   Expresses TPMV H glycoprotein in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV H glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 2 to 33 at N terminus deleted

   Promoter

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJC6-NADK2-3xFLAG

   Plasmid

   #233895

   Purpose

   lentiviral overexpression vector for NADK2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   NADK2 (NADK2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVTNT-T7-KPNA2

   Plasmid

   #26678

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 2 (KPNA2 Human)
   

   Use

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. SeAvs3

   Plasmid

   #188791

   Purpose

   Avs3 from Salmonella enterica NCTC13175

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   SeAvs3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pExp-Sumo

    Plasmid

    #112574

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCer-C3-Plk1-T210D

    Plasmid

    #68133

    Purpose

    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    T210D constitutively active version

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET41sIII_GST-ALC1

    Plasmid

    #173932

    Purpose

    Bacterial expression of ALC1

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1 (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB_TRE3G_scFV-sfGFP-Ezh2FL

    Plasmid

    #235575

    Purpose

    Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pBS mFgfr1 (CT#92)

    Plasmid

    #14005

    Depositor

    Cliff Tabin

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pWPI

    Plasmid

    #12254

    Purpose

    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EMCV IRES-EGFP cassette

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRcCMV myc-Plk1 K82R (Nigg LA184)

    Plasmid

    #41157

    Depositor

    Erich Nigg

    Article

    Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K82R

    Promoter

    CMV

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-STAT3mts

    Plasmid

    #195577

    Purpose

    Expresses STAT3 with mitochondrial targeting sequence

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Tags

    Cox8 presequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR_PGK_SNIPR_CD8alpha variant 2

    Plasmid

    #188362

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR221-NPC1_STOP

    Plasmid

    #161461

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LP110a

    Plasmid

    #90199

    Purpose

    pEGFP-N1 with cDNA encoding full length HsKIF13A

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13A (KIF13A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available Since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCS4 3XFLAG-PPARgamma2

    Plasmid

    #78770

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2 (Ppargc1a Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

29. p354_DG

    Plasmid

    #234697

    Purpose

    Golden gate domesticated pET28b for scarless cloning with His10-TEV

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-N-HA-NEK7

    Plasmid

    #75142

    Purpose

    Expresses N-terminal HA-tagged NEK7 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge