165,786 results

1. pMEL12

   Plasmid

   #107918

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-RfxCas13d-HA

   Plasmid

   #141320

   Purpose

   Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   HA

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO-GAlpha15-RLuc8

   Plasmid

   #140984

   Purpose

   Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha15-RLuc8 (GNA15 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-SPN_ARR wt (Shank3)

   Plasmid

   #175254

   Purpose

   SPN-ARR fragment in EGFP backbone for mammalian expression

   Depositor

   Johanna Ivaska

   Article

   Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

   Insert

   SPN-ARR fragment from SHANK3
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-P-RAN-H2A2B

   Plasmid

   #106412

   Purpose

   P-RAN-H2A2B (RANbody H2A2B::vHRP)

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

   Insert

   P-RAN-H2A2B
   

   Tags

   HA and His

   Expression

   Mammalian

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXR004: CasRx pre-gRNA cloning backbone

   Plasmid

   #109054

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ144

   Plasmid

   #69296

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-pcDNA3.1 Puro-CAG-Positron2

   Plasmid

   #239079

   Purpose

   Mammalian expression of positive-going voltage sensor

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2
   

   Expression

   Mammalian

   Mutation

   R78K N81D D92N W178F

   Promoter

   CAG

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gs(short)-CASE

    Plasmid

    #168124

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG1

    Expression

    Mammalian

    Mutation

    NLuc is inserted at N136/V137 within GNAS

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RFP-eNOS-NLS

    Plasmid

    #22496

    Depositor

    William Sessa

    Article

    Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

    Insert

    eNOS(G2A)-NLS
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. YCPlac33-sGFP-VRG4

    Plasmid

    #25447

    Depositor

    Benjamin Glick

    Article

    Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

    Insert

    Synthetic Green Fluorescent Protein - VRG4
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Expression

    Mammalian

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

    Plasmid

    #157625

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL1 (KIR2DL1 Human)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

    Plasmid

    #157624

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL4 (KIR2DL4 Human)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbS8k-RFP_N99D

    Plasmid

    #109410

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    N99D repA mutation to increase copy number

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-miRFP670nano3

    Plasmid

    #184664

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS mTSP-2

    Plasmid

    #12999

    Depositor

    Jack Lawler

    Insert

    TSP-2 (Thbs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pDONR P2R-P3-EYFP

    Plasmid

    #48351

    Purpose

    Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTW411d

    Plasmid

    #246585

    Purpose

    different combination of A. thaliana and N. tabacum Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    NtRaf1, NtRaf2, NtRbcX2, NtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CCR2-HiBiT Fusion Vector

    Plasmid

    #238615

    Purpose

    Express CCR2-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CCR2 (CCR2 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. Lenti NbALFA-miRFP680 hygro

    Plasmid

    #193969

    Purpose

    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-miRFP680
    

    Use

    Lentiviral

    Tags

    miRFP680

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

    Plasmid

    #237890

    Purpose

    Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET30a-GABAT

    Plasmid

    #239572

    Purpose

    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    4-aminobutyrate aminotransferase (gabT E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1

    Plasmid

    #237139

    Purpose

    Express MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MLL1 (KMT2A Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. NanoBRET MLL1/WDR5 Assay Acceptor Vector, WDR5

    Plasmid

    #237140

    Purpose

    Express WDR5-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. CL20_MSCV-NUP98::HOXA9-ires-GFP

    Plasmid

    #237484

    Purpose

    Express NUP98::HOXA9 in bicistronic retroviral vector

    Depositor

    Charles Mullighan

    Article

    Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

    Insert

    NUP98::HOXA9 (HOXA9, NUP98 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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