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Showing: 6641 - 6680 of 10097 results

  1. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  2. pT2/shαSMA/GFP4_Seq1/clone1

    Plasmid
    #206138
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA (Acta2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206138-plasmid-map-sequence-id-410128

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  3. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

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  4. DYSF-Exon36-del

    Plasmid
    #60190
    Purpose
    Plasmid contains human dysferlin with exon 36 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 36 has been deleted
    Promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    60190-plasmid-map-sequence-id-96285

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  5. DYSF-Exon9-del

    Plasmid
    #60180
    Purpose
    Plasmid contains human dysferlin with exon 9 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 9 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60180-plasmid-map-sequence-id-96272

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  6. DYSF-Exon34-del

    Plasmid
    #60188
    Purpose
    Plasmid contains human dysferlin with exon 34 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 34 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60188-plasmid-map-sequence-id-96283

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  7. DYSF-Exon45-del

    Plasmid
    #60196
    Purpose
    Plasmid contains human dysferlin with exon 45 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 45 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60196-plasmid-map-sequence-id-96290

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  8. DYSF-Exon49-del

    Plasmid
    #60197
    Purpose
    Plasmid contains human dysferlin with exon 49 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 49 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60197-plasmid-map-sequence-id-96291

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  9. DYSF-Exon7-del

    Plasmid
    #60178
    Purpose
    Plasmid contains human dysferlin with exon 7 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 7 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60178-plasmid-map-sequence-id-96267

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  10. DYSF-Exon43-del

    Plasmid
    #60195
    Purpose
    Plasmid contains human dysferlin with exon 43 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 43 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    60195-plasmid-map-sequence-id-96276

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  11. DYSF-Exon14-del

    Plasmid
    #60181
    Purpose
    Plasmid contains human dysferlin with exon 14 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 14 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60181-plasmid-map-sequence-id-96273

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  12. DYSF-Exon25-del

    Plasmid
    #60185
    Purpose
    Plasmid contains human dysferlin with exon 25 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 25 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60185-plasmid-map-sequence-id-96280

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  13. DYSF-Exon30-del

    Plasmid
    #60186
    Purpose
    Plasmid contains human dysferlin with exon 30 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 30 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60186-plasmid-map-sequence-id-96281

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  14. DYSF-Exon19-del

    Plasmid
    #60183
    Purpose
    Plasmid contains human dysferlin with exon 19 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 19 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60183-plasmid-map-sequence-id-96278

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  15. DYSF-Exon24-del

    Plasmid
    #60184
    Purpose
    Plasmid contains human dysferlin with exon 24 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 24 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60184-plasmid-map-sequence-id-96279

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  16. DYSF-Exon38-del

    Plasmid
    #60192
    Purpose
    Plasmid contains human dysferlin with exon 38 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 38 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60192-plasmid-map-sequence-id-96287

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  17. DYSF-Exon35-del

    Plasmid
    #60189
    Purpose
    Plasmid contains human dysferlin with exon 35 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 35 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60189-plasmid-map-sequence-id-96284

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  18. DYSF-Exon8-del

    Plasmid
    #60179
    Purpose
    Plasmid contains human dysferlin with exon 8 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 8 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60179-plasmid-map-sequence-id-96270

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  19. DYSF-Exon32-del

    Plasmid
    #60187
    Purpose
    Plasmid contains human dysferlin with exon 32 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 32 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60187-plasmid-map-sequence-id-96282

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  20. DYSF-Exon37-del

    Plasmid
    #60191
    Purpose
    Plasmid contains human dysferlin with exon 37 deleted. Deletion maintains the open reading frame.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 37 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60191-plasmid-map-sequence-id-96286

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  22. pGFP Cortactin

    Plasmid
    #50728
    Purpose
    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Cortactin (CTTN Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50728-plasmid-map-sequence-id-339652

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  23. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  27. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid
    #50458
    Purpose
    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    rM3D-mCherry
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50458-plasmid-map-sequence-id-162264

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  28. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Promoter
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  29. pFRT-TODestFLAGHA_RNAseH1

    Plasmid
    #65782
    Purpose
    expresses FLAGHA-tagged RNAsH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65782-plasmid-map-sequence-id-345165

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  30. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid
    #181980
    Purpose
    Human RUNX1C gene overexpression
    Depositor
    Jan-Henning Klusmann
    Insert
    RUNX1C (human) (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA (Human influenza hemagglutinin)
    Expression
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    181980-plasmid-map-sequence-id-455918

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  31. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122182-plasmid-map-sequence-id-237289

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  32. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122183-plasmid-map-sequence-id-241282

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  33. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid
    #122200
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9
    TagRFP-T
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122200-plasmid-map-sequence-id-236123

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  34. pHA AKT2 T309A/S474A

    Plasmid
    #60128
    Purpose
    expresses a mutant AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Serine 474 and Threonine 309 changed to alanines
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP

    Plasmid
    #181979
    Purpose
    Human RUNX1A gene overexpression
    Depositor
    Jan-Henning Klusmann
    Insert
    RUNX1A (human) (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA (Human influenza hemagglutinin)
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181979-plasmid-map-sequence-id-358973

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  38. pFRT-DestFLAGHA_RNAseH1

    Plasmid
    #65783
    Purpose
    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65783-plasmid-map-sequence-id-151430

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  39. pGFP CrkII

    Plasmid
    #50730
    Purpose
    mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    CrkII (CRK Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6641 - 6680 of 10097 results