4,178 results

1. pQCXIH-Myc-YAP-5SA

   Plasmid

   #33093

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Promoter

   CMV/MSV

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1a-Bmi1

   Plasmid

   #31783

   Depositor

   Xin Chen

   Article

   Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

   Insert

   Bmi1 (BMI1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SpyTagMBP

   Plasmid

   #35050

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyTagMBP
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Simple peptide tag added to the N-terminus of MBP…

   Promoter

   T7 promoter

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV/TO Puro empty (w175-1)

   Plasmid

   #17482

   Purpose

   3rd gen lentiviral empty control vector, CMV/TO promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-Stat3-C

   Plasmid

   #13373

   Purpose

   Retroviral expression of dominant active mouse Stat3

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Changed Alanine 661 to cysteine and asparagine 66…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-DsRed FP (pMIDsRed II)

   Plasmid

   #52110

   Purpose

   a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-DsRed

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5 human p27

   Plasmid

   #14049

   Depositor

   Joan Massague

   Article

   Luo et al Nature. 1995 May 11. 375(6527):159-61.

   Insert

   p27 (CDKN1B Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-B-GECO1

   Plasmid

   #32448

   Purpose

   Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   B-GECO1.0
   

   Expression

   Mammalian

   Mutation

   GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

   Promoter

   CMV

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-DN-hCUL4B-FLAG

   Plasmid

   #15822

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4B (CUL4B Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N594

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

    Plasmid

    #141390

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin5 (CUL5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. IKK-2 K44M

    Plasmid

    #11104

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K44M, kinase inactive

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDsRed2-Keap1

    Plasmid

    #21551

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    kelch-like ECH-associated protein 1 (KEAP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Aequorea Victoria, Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Cyclin D1

    Plasmid

    #19927

    Depositor

    Yue Xiong

    Article

    Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

    Insert

    Cyclin D1 (CCND1 Human)
    

    Expression

    Mammalian

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET9d-hnRNP-A1

    Plasmid

    #23026

    Depositor

    Douglas Black

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/HA-FLAG-RBP2

    Plasmid

    #14800

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. DsRed-Rab5 WT

    Plasmid

    #13050

    Depositor

    Richard Pagano

    Article

    Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

    Insert

    RAB5A (Canis familiaris) (RAB5A Canis familiaris)
    

    Tags

    DsRed2 and HA

    Expression

    Mammalian

    Mutation

    asparagine 3 changed to serine (neutral); serine …

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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