4,235 results

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PX404 Campylobacter jejuni Cas9

Plasmid

#68338

Purpose

Campylobacter jejuni Cas9

Depositor

Feng Zhang

Insert

CjCas9

Use

CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Tubulin

Plasmid

#36202

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Alpha-tubulin (TUBA1B Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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BE4

Plasmid

#100802

Purpose

Expresses BE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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1236 pcDNA3 Myr HA Akt3

Plasmid

#9017

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Tags

HA and Myr

Expression

Mammalian

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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pMIG-hSOX2

Plasmid

#17226

Depositor

George Daley

Article

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

SRY (sex determining region Y)-box 2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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mApple-pBAD

Plasmid

#54536

Purpose

Localization: Protein Expression Vector , Excitation: 568, Emission: 592

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

6xHis and mApple

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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V405 4HA-CKIe

Plasmid

#13724

Depositor

David Virshup

Article

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Tags

4 HA

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChETA-EYFP

Plasmid

#26968

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Has Service

AAV1, AAV5, and AAV9

Insert

hChR2(E123T/H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T, H134R

Promoter

EF1alpha

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-Q61L

Plasmid

#12974

Depositor

Gary Bokoch

Insert

Cdc42 constitutively active (CDC42 Human)

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a/Cas9-Cys

Plasmid

#53261

Purpose

Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

Depositor

Hyongbum Kim

Article

Ramakrishna et al Genome Res. 2014 Apr 2.

Insert

Cas9-Cys

Tags

6X His tag, HA epitope, and NLS

Expression

Bacterial

Mutation

Additional Cysteine is added to the C-terminal re…

Promoter

T7 Promoter

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP1

Plasmid

#52519

Purpose

For mammalian expression of the fluorescent voltage sensor ASAP1

Depositor

Michael Lin

Article

St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

Insert

Accelerated Sensor of Action Potentials 1

Expression

Mammalian

Promoter

CAG

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEZYflag

Plasmid

#18700

Purpose

pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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CMV::SypHy A4

Plasmid

#24478

Depositor

Leon Lagnado

Article

Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

Insert

SypHy (Syp Rat)

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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Lentiviral-FOP-dGFP-reporter

Plasmid

#14885

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

mutant LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pPRIME-TET-GFP-FF3

Plasmid

#11662

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-Lats1

Plasmid

#19053

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

**

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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