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We narrowed to 18,759 results for: nls

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Showing: 6681 - 6720 of 18759 results

  1. pSA128 A2UCOE-HBB_IVS2-Cas9-BFP

    Plasmid
    #249152
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-Cas9-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249152-plasmid-map-sequence-id-496394

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  9. P4-TadCBEd

    Plasmid
    #225143
    Purpose
    Packaging plasmid for TadCBEd in v3b PE-eVLP architecture
    Depositor
    David Liu
    Publication
    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4-TadCBEd
    Expression
    Mammalian
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225143-plasmid-map-sequence-id-445268

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  14. eGFP-SUN1

    Plasmid
    #213515
    Purpose
    Expresses human eGFP-SUN1 in mammalian cells
    Depositor
    Karim Mekhail
    Publication
    Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUN1 (SUN1 Human)
    Expression
    Mammalian
    Mutation
    SUN1 has a silent mutation on its gene (nucleotid…
    Promoter
    CMV
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213515-plasmid-map-sequence-id-422354

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  16. nLbCas12a

    Plasmid
    #213745
    Purpose
    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLbCas12a
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213745-plasmid-map-sequence-id-422202

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  19. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205412-plasmid-map-sequence-id-416556

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  25. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Publication
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  26. pPE4max-Blasticidin

    Plasmid
    #194905
    Purpose
    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.
    Depositor
    George Church
    Publication
    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BSD
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    194905-plasmid-map-sequence-id-383725

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  28. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  31. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon-optimized dCas12Max-CBE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  37. pGES401

    Plasmid
    #190198
    Purpose
    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection
    Depositor
    Yuefeng Guan
    Publication
    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190198-plasmid-map-sequence-id-371936

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  40. PB-PEmax NG

    Plasmid
    #187894
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, NG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187894-plasmid-map-sequence-id-370740

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Showing: 6681 - 6720 of 18759 results