We narrowed to 4,969 results for: mcs

1. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

   Plasmid

   #195543

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. t1-405

   Plasmid

   #166127

   Purpose

   For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   Tln1Head (1-405) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-405 only

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-MCU-mCerulean

   Plasmid

   #185809

   Purpose

   Human mitochondrial calcium uniporter with C-terminus mCerulean tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

   Plasmid

   #192185

   Purpose

   Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 light chain (HK02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.puro_shGLS

   Plasmid

   #110335

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Article

   Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

   Insert

   GLS glutaminase (GLS Human, Synthetic)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX/PD-L1 3SA

   Plasmid

   #121467

   Purpose

   Expression of GST-human PD-L1 in E. Coli

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S176A, T180A, S184A

   Promoter

   Tac

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS10-ribE-dddAI-FLAG

   Plasmid

   #186562

   Purpose

   Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   pSpac(hy)

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. t206-405

   Plasmid

   #166132

   Purpose

   For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-F2F3(206-405) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 206-405 only

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203219

   Purpose

   Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-QNZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgLATS1/2-1

    Plasmid

    #229431

    Purpose

    knockout of LATS1 and LATS2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgLATS1/2-1 (LATS1, LATS2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 G142D (GAT)+deltaV143-Y145

    Plasmid

    #212459

    Purpose

    Encodes SARS-CoV-2 spike G142D, V143-Y145 deletion for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike G142D, V143-Y145 deletion (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    G142D, V143-Y145 deletion

    Promoter

    CAG

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

    Plasmid

    #212504

    Purpose

    Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC) S373P (CCA) S375F (TTC) T376A (GCT)

    Plasmid

    #212493

    Purpose

    Encodes SARS-CoV-2 spike S371F, S373P, S375F, T376A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike S371F, S373P, S375F, T376A (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    S371F, S373P, S375F, T376A

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

    Plasmid

    #212498

    Purpose

    Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    Q493R, Q498R, N501Y, Y505H

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Spike-deltaC18 S371L (CTC) S373P (CCA) S375F (TTC)

    Plasmid

    #212467

    Purpose

    Encodes SARS-CoV-2 spike S371L, S373P, S375F for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike S371L, S373P, S375F (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    S371L, S373P, S375F

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

    Plasmid

    #212472

    Purpose

    Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    S477N, T478K, E484A

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist-SARS-CoV-2 Spike-deltaC18 N211I (ATT) deltaL212+InsertionR214-EPE

    Plasmid

    #212462

    Purpose

    Encodes SARS-CoV-2 spike N211I, L212 deletion, R214 insertion for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike N211I, L212 deletion, R214 insertion (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    N211I, L212 deletion, R214 insertion

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.621 (Mu)

    Plasmid

    #212452

    Purpose

    Encodes SARS-CoV-2 spike T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SARS-CoV-2 Spike-deltaC18 AY.4.2

    Plasmid

    #212457

    Purpose

    Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA) E484K (AAA)

    Plasmid

    #212439

    Purpose

    Encodes SARS-CoV-2 spike K417N, L452R, T478K, E484K for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike K417N, L452R, T478K, E484K (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    K417N, L452R, T478K, E484K

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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