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Showing: 5041 - 5070 of 12385 results

  1. pBSFI-myr-akt-T308A

    Plasmid
    #49189
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector
    Depositor
    Peter Vogt
    Article
    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    T308A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. USP04

    Plasmid
    #25223
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp04.001.229 (Usp4 Mouse)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pclbw-PAGFPomp25

    Plasmid
    #58427
    Purpose
    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    paGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    PAGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    cmv-bactin
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pFUSEss-CHIg-mG1_CH3-3

    Plasmid
    #105853
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    105853-plasmid-map-sequence-id-305783

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  7. mEos3.2-H1-10

    Plasmid
    #57457
    Purpose
    Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    H1 (H1f0 Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57457-plasmid-map-sequence-id-124124

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  9. pGL2

    Plasmid
    #48742
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +386)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    truncated promoter region (see pGL6)
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pD649-HAsp-mIslr2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157616
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    Islr2 (Islr2 Mouse)
    Use
    Tags
    Fc(DAPA)-Avi-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    157616-plasmid-map-sequence-id-381382

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  16. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    -
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124802-plasmid-map-sequence-id-244317

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  17. mCherry-ATG3-N-18

    Plasmid
    #54994
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG3 (ATG3 Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pGEX-MK1

    Plasmid
    #29638
    Depositor
    Bruce Tempel
    Insert
    Mk1 (Kcna1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  23. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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  27. Mouse-Dysferlin-Transcript-Variant2

    Plasmid
    #60215
    Purpose
    Mouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (Dysf Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60215-plasmid-map-sequence-id-96322

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  28. pDest27-mBIN1+17

    Plasmid
    #61096
    Purpose
    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. p152 pWZL-EGFP RanBP10

    Plasmid
    #11098
    Depositor
    Ramesh Shivdasani
    Insert
    RanBP10 (Ranbp10 Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
Showing: 5041 - 5070 of 12385 results