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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Article

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCRY2PHR-mCherryN1

Plasmid

#26866

Purpose

Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2PHR (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

Plasmid

#185800

Purpose

one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DNAseI

Plasmid

#182408

Purpose

Bacterial expression of DNaseI

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DNase I (DNASE1 Bovine)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFD116

Plasmid

#124769

Purpose

pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Inserts

Ptet
dcas9
PpflB sgRNA BsaI

Use

CRISPR

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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FH-TET3-pEF

Plasmid

#49446

Purpose

This plasmid expresses full-length human TET3.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

Tet methylcytosine dioxygenase 3 (TET3 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Expression

Bacterial

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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