We narrowed to 4,936 results for: AAT

Showing: 641 - 680 of 4936 results

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MLSE-shTAF12.364

Plasmid

#105577

Purpose

retrovirally express TAF12 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTAF2.4392

Plasmid

#105562

Purpose

retrovirally express TAF2 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF2 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTAF12.364

Plasmid

#105559

Purpose

retrovirally express of TAF12 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LEP-shTAF12.376

Plasmid

#105560

Purpose

retrovirally express TAF12 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA1_pNeurog2-Luciferase reporter

Plasmid

#64160

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for pNeurog2-Luciferase reporter

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4T

Plasmid

#62262

Purpose

expression of A4T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-c-let-7-LP4B-IRES-EGFP

Plasmid

#37647

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-c-let-7-LP4A-IRES-EGFP

Plasmid

#37646

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP6-IRES-EGFP

Plasmid

#37645

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP6 - AATATTACCA=>AATATTACAT

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP5-IRES-EGFP

Plasmid

#37644

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP5 - AATATTACCA=>AATATTTACA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP4-IRES-EGFP

Plasmid

#37643

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP3-IRES-EGFP

Plasmid

#37642

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP3 - AATATTACCA=>AAATTTACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Mutation

Codon Optimised wtCNa

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Article

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-3'UTR

Plasmid

#136038

Purpose

G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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TRIN-shMYB.2572

Plasmid

#105574

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB shRNA #2572

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available Since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBA-mCherry-EGFP-PIM

Plasmid

#111758

Purpose

Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

chicken beta-actin

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pQCXIP TBRII

Plasmid

#19147

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

Transforming growth factor, beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
GFP-PKI WT

Plasmid

#118300

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI wt

Tags

Flag and GFP

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ23119-sgRNA

Plasmid

#113654

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter J23119 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-CAPRIN1-3'UTR

Plasmid

#136055

Purpose

CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-AsCas12a_crRNA-site4 (MSP3511)

Plasmid

#160139

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

Plasmid

#197952

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_834

Plasmid

#216087

Purpose

Cas9 [Sp] knockout targeting CD59, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD59 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFG.CNa12_opt.IRES.eGFP

Plasmid

#22490

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 12 (PPP3CA Human)

Use

Retroviral

Mutation

T351E; L354A

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-SFXN3

Plasmid

#110637

Purpose

Retroviral expression of Flag-SFXN3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN3 (SFXN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 4,936 results for: AAT