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Showing: 641 - 680 of 3510 results

  5. pBA-mCherry-EGFP-PIM

    Plasmid
    #111758
    Purpose
    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    chicken beta-actin
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111758-plasmid-map-sequence-id-222504

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  6. pGW1-mCherry-EGFP-PIM

    Plasmid
    #111759
    Purpose
    High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    CMV
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111759-plasmid-map-sequence-id-222507

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  11. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Eric Senning
    Article
    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Use
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220307-plasmid-map-sequence-id-436649

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  16. pFEndoNA

    Plasmid
    #174724
    Purpose
    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174724-plasmid-map-sequence-id-343066

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  17. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid
    #197952
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197952-plasmid-map-sequence-id-387870

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  21. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  24. pJ23119-sgRNA

    Plasmid
    #113654
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    promoter J23119 and sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113654-plasmid-map-sequence-id-217170

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Showing: 641 - 680 of 3510 results