Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,431 results for: BRE

Clear keywords
Showing: 801 - 850 of 4431 results

  1. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Article
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Human, Phage, Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40591-plasmid-map-sequence-id-302078

    Enlarge

  2. A1-LCD+7R+12D

    Plasmid
    #178678
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+12D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, twelve Glu residues add…
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178678-plasmid-map-sequence-id-357023

    Enlarge

  7. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid
    #196092
    Purpose
    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 96-285 (predicted disordered …
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196092-plasmid-map-sequence-id-384633

    Enlarge

  8. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid
    #196094
    Purpose
    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 638-925 (predicted disordered…
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196094-plasmid-map-sequence-id-384649

    Enlarge

  9. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid
    #196095
    Purpose
    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Contains only AA 619-925 (NLS and predicted disor…
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196095-plasmid-map-sequence-id-384632

    Enlarge

  10. A1-LCD-2R-2K+3D

    Plasmid
    #178680
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-2R+2K+3D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    two Arg residues removed, two Lys added, three As…
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178680-plasmid-map-sequence-id-356258

    Enlarge

  11. A1-LCD-9F+3Y

    Plasmid
    #178663
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+3Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, three Tyr added
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178663-plasmid-map-sequence-id-356435

    Enlarge

  12. A1-LCD-14N+14Q

    Plasmid
    #178692
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-14N+14Q (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    fourteen Gln residues replaced with Asn
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178692-plasmid-map-sequence-id-356415

    Enlarge

  14. A1-LCD+7K+12D

    Plasmid
    #178679
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7K+12D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Lys residues added, twelve Glu residues add…
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178679-plasmid-map-sequence-id-356398

    Enlarge

  15. A1-LCD-30G+30S

    Plasmid
    #178684
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178684-plasmid-map-sequence-id-356310

    Enlarge

  16. A1-LCD+23G-23S

    Plasmid
    #178685
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178685-plasmid-map-sequence-id-356307

    Enlarge

  17. A1-LCD-4R-2K+5D

    Plasmid
    #178681
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-4R+2K+5D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    four Arg residues removed, two Lys added, five As…
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178681-plasmid-map-sequence-id-356295

    Enlarge

  18. A1-LCD-9F+6Y

    Plasmid
    #178661
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+6Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, six Tyr added
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178661-plasmid-map-sequence-id-356293

    Enlarge

  19. A1-LCD-8F+4Y

    Plasmid
    #178662
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-8F+4Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    eight Phe residues removed, four Tyr added
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178662-plasmid-map-sequence-id-356201

    Enlarge

  20. A1-LCD+7R+10D

    Plasmid
    #178677
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+10D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, ten Glu residues added
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178677-plasmid-map-sequence-id-356191

    Enlarge

  21. A1-LCD-12F+12Y

    Plasmid
    #178659
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD -12F+12Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twelve Phe residues replaced with Tyr
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178659-plasmid-map-sequence-id-356187

    Enlarge

  22. A1-LCD-23S+23T

    Plasmid
    #178693
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-23S+23T (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Thr reisudes replaced with Ser
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178693-plasmid-map-sequence-id-356186

    Enlarge

  24. A1-LCD-20G+20S

    Plasmid
    #178683
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178683-plasmid-map-sequence-id-356148

    Enlarge

  30. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available Since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

    Enlarge

  32. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available Since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

    Enlarge

  33. CIRTS-FTO-control

    Plasmid
    #216859
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216859-plasmid-map-sequence-id-430696

    Enlarge

  35. CIRTS-YTHDF2-control

    Plasmid
    #216857
    Purpose
    CIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-YTHDF2-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    216857-plasmid-map-sequence-id-448432

    Enlarge

  42. CIRTS-YTHDF2-Malat1

    Plasmid
    #216858
    Purpose
    CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216858-plasmid-map-sequence-id-443348

    Enlarge

  43. CIRTS-FTO-Malat1

    Plasmid
    #216860
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-Malat1 gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    216860-plasmid-map-sequence-id-434854

    Enlarge

  44. A1-LCD-30G+30S-12F+12Y

    Plasmid
    #178687
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, twelve Phe…
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178687-plasmid-map-sequence-id-357019

    Enlarge

  45. A1-LCD-30G+30S+7F-7Y

    Plasmid
    #178686
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, seven Tyr …
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178686-plasmid-map-sequence-id-357017

    Enlarge

  46. A1-LCD-20G+20S+7F-7Y

    Plasmid
    #178688
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, seven Tyr …
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178688-plasmid-map-sequence-id-356407

    Enlarge

  47. A1-LCD-14N-4Q+18G

    Plasmid
    #178694
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-14N-4Q+18G (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    fourteen Gln residues removed, four Asn removed, …
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178694-plasmid-map-sequence-id-356315

    Enlarge

  48. A1-LCD-20G+20S-12F+12Y

    Plasmid
    #178689
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, twelve Phe…
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178689-plasmid-map-sequence-id-356275

    Enlarge

  49. A1-LCD+23G-23S-12F+12Y

    Plasmid
    #178691
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, twel…
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178691-plasmid-map-sequence-id-356195

    Enlarge

  50. A1-LCD+23G-23S+7F-7Y

    Plasmid
    #178690
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, seve…
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178690-plasmid-map-sequence-id-356145

    Enlarge

Showing: 801 - 850 of 4431 results