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  3. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

    Plasmid
    #163719
    Purpose
    Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine
    Depositor
    Matthew Bennett
    Article
    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25
    Insert
    araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163719-plasmid-map-sequence-id-323479

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  9. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid
    #48992
    Purpose
    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Inserts
    cre recombinase
    Luciferase
    Use
    Luciferase; Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pBEG R2-iPuro-L3 #BGV205

    Plasmid
    #48987
    Purpose
    Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    puromycin resistance
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    NA
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pPTG-GG-sg2+sg3

    Plasmid
    #127199
    Purpose
    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA
    Use
    Empty sgrna plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127199-plasmid-map-sequence-id-248025

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  14. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid
    #67645
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.
    Depositor
    Stefan Stamm
    Article
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' and 3' splice sites mutations
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67645-plasmid-map-sequence-id-116914

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  15. pBEG R2-eGFP-L3 #BGV201

    Plasmid
    #48979
    Purpose
    Supplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    eGFP
    Use
    Gateway cloning vector
    Tags
    IRES (strong)
    Expression
    Mutation
    Promoter
    NA
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pBEG R2-iNeo-L3 #BGV207

    Plasmid
    #48986
    Purpose
    Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    neomycin resistance
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    NA
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pBEG R2-eCFP-L3 #BGV202

    Plasmid
    #48977
    Purpose
    Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    eCFP
    Use
    Gateway cloning vector
    Tags
    IRES (strong)
    Expression
    Mutation
    Promoter
    NA
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pMU103

    Plasmid
    #73934
    Purpose
    This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.
    Depositor
    Zhanyuan Zhang
    Article
    Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d
    Insert
    bar and GUS cassettes
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    73934-plasmid-map-sequence-id-139933

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  31. JD179

    Plasmid
    #229496
    Purpose
    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, L4-22k/33k, VA RNA I
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    229496-plasmid-map-sequence-id-455847

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  34. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  35. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193786-plasmid-map-sequence-id-389837

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  36. pTA1cI-T3

    Plasmid
    #193789
    Purpose
    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193789-plasmid-map-sequence-id-389812

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  38. pENTR-D-kcnj13

    Plasmid
    #173143
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173143-plasmid-map-sequence-id-343318

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  39. pENTR-D-kcnj1b

    Plasmid
    #173126
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj1b (kcnj1b Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173126-plasmid-map-sequence-id-342841

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  40. pDONR221-kcnj8

    Plasmid
    #173134
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173134-plasmid-map-sequence-id-343157

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  41. pREG(R1-R4) #BGV059

    Plasmid
    #48991
    Purpose
    Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pCAGGS-FuG-C

    Plasmid
    #67512
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type C envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al Hum Gene Ther. 2011 Dec;22(12):1511-23.
    Insert
    Fusion Glycoprotein type C
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67512-plasmid-map-sequence-id-116094

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  45. pCAGGS-FuG-B

    Plasmid
    #67523
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d
    Insert
    Fusion Glycoprotein type B
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67523-plasmid-map-sequence-id-116136

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  46. pRRL.PPT.SFFV.MCS.IRES.dTomato

    Plasmid
    #171174
    Purpose
    Lentiviral expression vector co-expressing dTomato
    Depositor
    Dirk Heckl
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171174-plasmid-map-sequence-id-344454

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  47. 1352wUV2-kpn1-bamH1-stop

    Plasmid
    #20704
    Depositor
    Scot Wolfe
    Insert
    4 finger ZFP
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20704-plasmid-map-sequence-id-9627

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  48. pDS2149

    Plasmid
    #184275
    Purpose
    Tet-on genome insertion system
    Depositor
    Dominique Sanglard
    Insert
    CatrTA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    184275-plasmid-map-sequence-id-361619

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  49. pwAB-erg10erg13

    Plasmid
    #227346
    Purpose
    Overexpression of the erg10 and erg13 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the erg10 and erg13 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227346-plasmid-map-sequence-id-458526

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  50. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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Showing: 801 - 850 of 158806 results