Skip to main content
Addgene
Showing: 801 - 850 of 57718 results

  2. pLV-mRFP1-Human UTROPHIN_1-261

    Plasmid
    #225052
    Purpose
    Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and RFP1 (UTRN Homo Sapiens, Human)
    Use
    Lentiviral
    Tags
    Human UTROPHIN 1-261 and mRFP1
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26739-R…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    225052-plasmid-map-sequence-id-446521

    Enlarge

  3. pLV-EGFP-Human-UTROPHIN_1-261

    Plasmid
    #222393
    Purpose
    Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and EGFP (UTRN Homo Sapiens, Human)
    Use
    Lentiviral
    Tags
    EGFP and Human UTROPHIN 1-261
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26737-G…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222393-plasmid-map-sequence-id-446518

    Enlarge

  10. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114194-plasmid-map-sequence-id-224256

    Enlarge

  18. pLenti-gRNA-puro

    Plasmid
    #180426
    Purpose
    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    gRNAscr
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    180426-plasmid-map-sequence-id-354805

    Enlarge

  19. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

    Enlarge

  25. WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis

    Plasmid
    #228660
    Purpose
    Bacterial expression of the fusion of the NS2B-NS3 protease from West Nile virus with an H163A - catalytically inactive mutation for xxxxx
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    West Nile portion of NS2B protease fused to NS3
    Use
    Tags
    His-Twin Strep-Tev
    Expression
    Bacterial
    Mutation
    H163A - catalytically inactive/West Nile Virus NS…
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228660-plasmid-map-sequence-id-461601

    Enlarge

  34. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

    Enlarge

  41. TPST2-EGFP

    Plasmid
    #66618
    Purpose
    Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)
    Depositor
    David Stephens
    Article
    Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do
    Insert
    TPST2 (TPST2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Insert contains T370A relative to NM_001008566.1
    Promoter
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

    Enlarge

Showing: 801 - 850 of 57718 results