Addgene

We narrowed to 1,772 results for: rpe

Showing: 801 - 850 of 1772 results

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pLC242-GFP-Sestrin2

Plasmid

#100519

Purpose

Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SESN2 (SESN2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Use

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pEasy Flox

Plasmid

#11725

Depositor

Klaus Rajewsky

Article

Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

Insert

pEasyFlox

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Msx1

Plasmid

#34998

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Homeobox protein MSX-1 (Msx1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-Cer.zf1

Plasmid

#61389

Purpose

mRNA synthesis of cerulean codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cer.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-USP44-HIS

Plasmid

#43894

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only
Drp1(A395D)-StrepII

Plasmid

#174430

Purpose

Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

StrepII

Expression

Bacterial

Mutation

A395D

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Drp1-StrepII

Plasmid

#174428

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-ICP8 (pSLIK1)

Plasmid

#113856

Purpose

For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pATD

Plasmid

#141012

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HSV-TK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p6897 PHAGE-P CMVT N-HA-hE6AP III wt

Plasmid

#37605

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV tet regulatable

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pRSLeu2d-BISopt

Plasmid

#35524

Depositor

Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

P GAL 1

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
p6896 PHAGE-P CMVT N-HA-hE6AP II wt

Plasmid

#37603

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II wt (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GST-Q11

Plasmid

#185790

Purpose

Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-Q11

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-BCA-Pep-H

Plasmid

#185782

Purpose

Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-Pep-H

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-BFLF1

Plasmid

#162626

Purpose

Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII p16

Plasmid

#16455

Depositor

Bert Vogelstein

Article

Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

Insert

p16 (CDKN2A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
UL79-3xFLAG pCDNA4

Plasmid

#120374

Purpose

Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.sfGFP-RepEntry.iPAC

Plasmid

#100893

Purpose

Lentiviral CRISPR-Cas9 Reporter

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Inserts

sfGFP
PAC

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-USP44

Plasmid

#43893

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 or T7

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS337-USP44-FLAG

Plasmid

#43905

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Baculoviral expression

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
p6672 MSCV-IP N-HAonly 52E6

Plasmid

#44136

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV52 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p6869 pOZ-N puro HA-HPV18 E2

Plasmid

#35134

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E2 (E2 HPV18)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pJAID8Z104

Plasmid

#165056

Purpose

Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p6666 MSCV-IP N-HAonly 18E6

Plasmid

#44137

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV18 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
p6674 MSCV-IP N-HAonly 6bE6

Plasmid

#44139

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV6b E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
p6664 MSCV-IP N-HAonly 45E6

Plasmid

#44138

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV45 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pFS331-USP44-catDead-HIS

Plasmid

#43903

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

C282A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
ZP43 Wnt8 3' orf1

Plasmid

#16899

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 3' orf1 (wnt8a Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Use

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ZE19 wnt8 pBand3-5

Plasmid

#17053

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 pBand3-5 (wnt8a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p6671 MSCV-IP N-HAonly 38E6

Plasmid

#44144

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV38E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
ZE20 wnt8 pBand1-2

Plasmid

#17054

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 pBand1-2 (wnt8a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p6619 MSCV-IP N-HAonly UBE3AI

Plasmid

#35015

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

UBE3AI

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUB1

Plasmid

#208813

Purpose

Expresses arabinose-inducible JUB1-derived artificial transcription factor in bacterial cell

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

JUB1 TF

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Arabinsoe-inducible promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Article

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac WT SOX

Plasmid

#192055

Purpose

Expression vector for WT SOX

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX (ORF37)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-eGFP-Pep-H

Plasmid

#185788

Purpose

Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-Pep4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2368-Tier1-PhCMV_VP64

Plasmid

#169569

Purpose

Tier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2367-Tier1-PhCMV_VP16

Plasmid

#169568

Purpose

Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID6JY

Plasmid

#165049

Purpose

Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID8Z105

Plasmid

#165057

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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