We narrowed to 12,164 results for: 112

1. pNAS1b split mCherry 14-3-3β ctrl

   Plasmid

   #112031

   Purpose

   Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
   

   Tags

   Split mCherry

   Expression

   Bacterial

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOP3MT

   Plasmid

   #112601

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   Avi - 8His-MBP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTEAD1.1.0-gDNA

   Plasmid

   #112425

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

   Depositor

   Kevin White

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-BE4RA-P2A-Puro

   Plasmid

   #112672

   Purpose

   Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE4RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   EF1s

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMito.iGABASnFR.F102G.mRuby3

   Plasmid

   #112180

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   F102G

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6 MCV LTco S239A

   Plasmid

   #112194

   Purpose

   Expression of MCPyV LT mutant (Ser 239 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-PpiB

   Plasmid

   #112572

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-PpiB and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

   Plasmid

   #112170

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   mRuby3

   Mutation

   F102G

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112210

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-PKCg-mEGFP-N1

    Plasmid

    #112270

    Purpose

    IDOCKSgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLBH546_Tet-Cas14b1Locus

    Plasmid

    #112503

    Purpose

    Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CytERM_3xmTurquoise2

    Plasmid

    #112958

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Insert

    CytERM
    

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xmTurquoise2_UtrCH

    Plasmid

    #112963

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    UtrCH
    

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. At1R-P2A-mCherry

    Plasmid

    #112934

    Purpose

    Plasmid co-producing the AT1R receptor and a mCherry

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    AT1R
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-Neo-JAM-A

    Plasmid

    #112965

    Purpose

    To express feline Jam-A transiently in cell lines that do not naturally express the receptor.

    Depositor

    John S. L. Parker

    Article

    Lu et al J Virol. 2018 Mar 28;92(8):e00035-18. do

    Insert

    Junctional Adhesion Molecule type A (JAM-A) (F11R Felis catus)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112209

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A,C710S

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ikaros

    Plasmid

    #11261

    Depositor

    Lisa Steiner

    Article

    Willett et al Dev Dyn. 2001 Dec . 222(4):694-8.

    Insert

    ikaros (ikzf1 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pLBH547_Tet-Cas14b2Locus

    Plasmid

    #112504

    Purpose

    Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b2
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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