We narrowed to 1,037 results for: David Sabatini

1. pLC242-GFP-SAMTOR

   Plasmid

   #100520

   Purpose

   Expresses GFP tagged SAMTOR in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SAMTOR (BMT2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LentiCRISPR-sgSHOC2-2

   Plasmid

   #86129

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   sgRNA 2 targeting SHOC2 (SHOC2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPR - DDX3Y sgRNA 1

   Plasmid

   #70656

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. HA Raptor mutant 1

   Plasmid

   #8470

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   194YDC196-AAA (Y194A, D195A, C196A)

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pRK5 Flag Grb10 9A long

   Plasmid

   #37485

   Depositor

   David Sabatini

   Article

   Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

   Insert

   Grb10 9A (GRB10 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   isoform A, 594aa, S104A S150A T155A S158A S426A S…

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pMXs-C3orf17-flag

   Plasmid

   #70646

   Purpose

   Expresses C3orf17 in mammalian cells with FLAG-tag epitope.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   C3orf17 (NEPRO Human)
   

   Use

   Retroviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMXs-C9orf114-gfp

   Plasmid

   #70266

   Purpose

   Expresses C9orf114 in mammalian cells with GFP reporter.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   C9orf114 (SPOUT1 )
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLJC6-Flag CASTOR1

   Plasmid

   #84496

   Purpose

   Lentiviral expression of CASTOR1

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   sestrin2

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. HA Raptor mutant 9

   Plasmid

   #8476

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pMXs_FLAG-ODC1

    Plasmid

    #133256

    Purpose

    Express FLAG-tagged ODC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC1 (ODC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. shGLDC-DOX

    Plasmid

    #72883

    Purpose

    dox inducible knockdown

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    RNAi hairpin against GLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCHA1.1-Rap2a-V5

    Plasmid

    #149509

    Purpose

    Stable expression of human Rap2a.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    RAP2 (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA Raptor mutant 4

    Plasmid

    #8474

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    391SQ392-PA (S391P, Q392A)

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLentiCRISPRv1-sgSHMT1-G3

    Plasmid

    #83903

    Purpose

    stable knockout

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Serine hydroxymethyltransferase 1 (SHMT1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-TAP Raptor

    Plasmid

    #12587

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Aug 15. ():.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pMXs_FLAG-SFXN2

    Plasmid

    #110636

    Purpose

    Retroviral expression of Flag-SFXN2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN2 (SFXN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMXs_FLAG-SFXN4

    Plasmid

    #110638

    Purpose

    Retroviral expression of Flag-SFXN4 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN4 (SFXN4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-FLAG-Sestrin2-E451A

    Plasmid

    #73677

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only