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pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5

Plasmid

#104842

Purpose

b2AR-SPARK TF component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

hLOV1-TEVcs-FLAG-GAL4-V5

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPotef-LN22*-3xGNLS-cbx

Plasmid

#86780

Purpose

Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

Depositor

Michael Feldbrügge

Article

Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

Insert

LN*

Use

Ustilago maydis expression

Tags

Gfp

Expression

Bacterial

Mutation

Promoter

Potef

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-4

Plasmid

#61746

Purpose

express N-terminally FLAG tagged human chimera USP13/5-4

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-4 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-716aa) fused to USP13 (722-864aa)

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-3

Plasmid

#61745

Purpose

express N-terminally FLAG tagged human chimera USP13/5-3

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-3 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-628aa) fused to USP13 (653-864aa)

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-1

Plasmid

#61743

Purpose

express N-terminally FLAG tagged human chimera USP13/5-1

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-1 chimera (USP5, USP13 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-185aa) fused to USP13 (197-864aa)

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Plasmid

#104844

Purpose

b2AR-SPARK TF component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1A2QS

Plasmid

#138268

Purpose

MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity

Depositor

Xiao Wang

Article

Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Inserts

AraC-TetR operon
LuxR-LacI operon
mCherry
GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Para/lac and Plux/tet

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMK110

Plasmid

#99535

Purpose

contains bgl operon sequence for E. coli in vitro transcription template

Depositor

Robert Landick

Article

Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

Insert

E. coli bgl antisense region containing bglG

Use

Tags

None

Expression

Mutation

Promoter

lambda Pr promoter used for in vitro transcriptio…

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-Emi1_control UTRs

Plasmid

#67925

Purpose

Expresses mCherry-Emi1 with control UTRs in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-Emi1 (FBXO5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.21

Plasmid

#62875

Purpose

Bacterial expression of MBP-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Use

Tags

His/HA

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Use

Tags

MBP

Expression

Bacterial

Mutation

K196R

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pJW4.34

Plasmid

#131370

Purpose

expression of GST:MEG-4 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-4 (C36C9.1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.33

Plasmid

#131369

Purpose

expression of GST:MEG-3 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-3 (meg-3 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.15

Plasmid

#131373

Purpose

expression of MBP:PAA-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

paa-1 (paa-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.31

Plasmid

#131371

Purpose

expression of GST:MEG-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-1 (meg-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.13

Plasmid

#131375

Purpose

expression of MBP:PPTR-2 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-2 (pptr-2 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.12

Plasmid

#131374

Purpose

expression of MBP:PPTR-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-1 (pptr-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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