We narrowed to 1,418 results for: RCA

1. pRCA594 - pBA904 Puro-T2A-BFP

   Plasmid

   #238170

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFR-mTq2-antiFRET-P2A-RCaMP1h

   Plasmid

   #191467

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   RCaMP1h
   

   Use

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOttc1494 - pAAV CaMKII SERCaMP-No Tag

   Plasmid

   #192601

   Purpose

   An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

   Depositor

   Brandon Harvey

   Insert

   SERCaMP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2Aneo-mRFP-FKBP-mSos1-linkercat

   Plasmid

   #173858

   Purpose

   Encoding a part of the rapamycin-induced Ras activation system.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   mRFP-FKBP-mSos1-linkercat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS (B) flag Radical Fringe (CT#472)

   Plasmid

   #13983

   Depositor

   Cliff Tabin

   Article

   Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

   Insert

   Radical Fringe (RFNG Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pDEST-mCherry-LacR-BRCA1 1366-1437

   Plasmid

   #71118

   Purpose

   mammalian expession of mCherry-LacR-BRCA1 1366-1437

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   contains amino acids 1366-1437 of BRCA1

   Promoter

   CMV/TO

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC031 - RcrCas13a from Rhodobacter capsulatus R121

   Plasmid

   #91921

   Purpose

   Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcrCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB CAG SMARCA4 T910M-IRES-EGFP

   Plasmid

   #153949

   Purpose

   piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T910M

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-FRT/T0-GFP-BRCA1 1364-1437

   Plasmid

   #71119

   Purpose

   Mammalian expression of GFP-BRCA1 1364-1437

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 1364-1437 of BRCA1

   Promoter

   CMV/TO

   Available since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

    Plasmid

    #200503

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-NRCAM-COMP5AP-AviTag-9xHis

    Plasmid

    #157597

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NRCAM (NRCAM Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-NRCAM-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157033

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NRCAM (NRCAM Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS6/U6

    Plasmid

    #15173

    Depositor

    Connie Cepko

    Article

    Harpavat et al BMC Dev Biol. 2006 . 6():2.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pDONR-U6gRNA-PGKpuro2APDGFB

    Plasmid

    #136409

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BbsI restriction site in the PDGFB sequence (Glu1…

    Promoter

    U6, PDK

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid

    #136405

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BbsI restriction site at position 437 was removed…

    Promoter

    U6, PDK

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid

    #131753

    Purpose

    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Inserts

    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    

    Use

    Standard for qrt-pcr

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP293

    Plasmid

    #204228

    Purpose

    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Inserts

    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1 and Saccharomyces c…

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR1055

    Plasmid

    #208741

    Purpose

    Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PHrcA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PHrcA

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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