We narrowed to 3,098 results for: actin

1. pDestTol2-actinb-kcnj13-EGFP

   Plasmid

   #164940

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME mKate2-dynactin1-811

   Plasmid

   #105971

   Purpose

   entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   dynactin1-811 (DCTN1 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   contains only amino acids 1-811

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nano-lantern-Actin/pEGFP-C1

   Plasmid

   #51972

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. hsp70: mKate2 dynactin1-811

   Plasmid

   #105970

   Purpose

   heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   dynactin1-811 (DCTN1 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   contains only amino acids 1-811

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin-BAF53(b+a)-ires-EGFP

   Plasmid

   #25462

   Depositor

   Jerry Crabtree

   Article

   Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

   Insert

   BAF53(b+a)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   3' of mBAF53b deleted and fused to 3' o…

   Promoter

   Available since

   Sept. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Actin-BAF53(a+b)-ires-EGFP

   Plasmid

   #25461

   Depositor

   Jerry Crabtree

   Article

   Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

   Insert

   BAF53(a+b)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   3' of mBAF53a deleted and fused to 3' o…

   Promoter

   Available since

   Sept. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ACTIN-Cxcl5-PGK-Cre

   Plasmid

   #110278

   Purpose

   Bicistronic lentivirus expressing Cxcl5 and Cre

   Depositor

   Trudy Oliver

   Article

   Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

   Inserts

   chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
   cyclization recombinase
   

   Use

   Cre/Lox and Lentiviral

   Tags

   No fusion genes

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   B-actin and Pgk

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pENTR-actin-G168D,Y169D

   Plasmid

   #118384

   Purpose

   Gateway compatible donor vector with actin-G168D,Y169D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   G168D,Y169D

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. psLIB TRE mTFP1-actin

   Plasmid

   #229970

   Purpose

   encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid

    #231548

    Purpose

    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid

    #196856

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacking the centrosome-targeting domain (CTD)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBactin-Nedd1-mOrange2-rCM1

    Plasmid

    #196861

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BAF53b + GFP/p-Actin-IRES

    Plasmid

    #197297

    Purpose

    Plasmid expressing an antigen targeting BAF53b

    Depositor

    Jerry Crabtree

    Insert

    BAF53b (Actl6b Mouse)
    

    Use

    Tags

    GFP (GFP observed)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid

    #191336

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    motor domain aa 1-405

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid

    #191333

    Purpose

    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBetaActin-FRB-dCer3-CAAX

    Plasmid

    #191326

    Purpose

    Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FRB-dCer-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid

    #191335

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid

    #191331

    Purpose

    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge