We narrowed to 3,232 results for: actin

1. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTag-eGFP-CAAX-B-actin

   Plasmid

   #117816

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTag-NLS-TagRFP-B-actin

   Plasmid

   #117806

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGTag-NLS-eGFP-B-actin

   Plasmid

   #117812

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. eTC GFP ß-actin ΔZip

   Plasmid

   #27124

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   This construct lacks β-actin 3′UTR and therefore …

   Available Since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mutant Human Cortactin-GFP

   Plasmid

   #71365

   Purpose

   mammalian expression of mutant human Cortactin-GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   cortactin (CTTN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutation present at 72nd nucleotide

   Promoter

   CMV

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Actin-53b-ires-EGFP

   Plasmid

   #25459

   Depositor

   Jerry Crabtree

   Article

   Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

   Insert

   BAF53b (Actl6b Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Contactin/F3 [K73/20R]

   Plasmid

   #177447

   Purpose

   Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBetaActin-KIF13aMotor-mScarlet-FKBP

   Plasmid

   #191337

   Purpose

   Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   KIF13aMotor-mScarlet-FKBP (KIF13A Human)
   

   Expression

   Mammalian

   Mutation

   motor domain aa 1-639

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2-actinb-kcnj13-EGFP

    Plasmid

    #164940

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME mKate2-dynactin1-811

    Plasmid

    #105971

    Purpose

    entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    dynactin1-811 (DCTN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    contains only amino acids 1-811

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nano-lantern-Actin/pEGFP-C1

    Plasmid

    #51972

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. hsp70: mKate2 dynactin1-811

    Plasmid

    #105970

    Purpose

    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    dynactin1-811 (DCTN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    contains only amino acids 1-811

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Actin-BAF53(b+a)-ires-EGFP

    Plasmid

    #25462

    Depositor

    Jerry Crabtree

    Article

    Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

    Insert

    BAF53(b+a)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    3' of mBAF53b deleted and fused to 3' o…

    Available Since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. Actin-BAF53(a+b)-ires-EGFP

    Plasmid

    #25461

    Depositor

    Jerry Crabtree

    Article

    Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

    Insert

    BAF53(a+b)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    3' of mBAF53a deleted and fused to 3' o…

    Available Since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACTIN-Cxcl5-PGK-Cre

    Plasmid

    #110278

    Purpose

    Bicistronic lentivirus expressing Cxcl5 and Cre

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

    Inserts

    chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
    cyclization recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    No fusion genes

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    B-actin and Pgk

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pENTR-actin-G168D,Y169D

    Plasmid

    #118384

    Purpose

    Gateway compatible donor vector with actin-G168D,Y169D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    G168D,Y169D

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. psLIB TRE mTFP1-actin

    Plasmid

    #229970

    Purpose

    encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mTFP1-actin
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid

    #196856

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Mutation

    Lacking the centrosome-targeting domain (CTD)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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