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pKNOCK-Cm

Plasmid

#46261

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Publication

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Publication

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGD-p19

Plasmid

#196326

Purpose

35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

Depositor

Zhenghe Li

Publication

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tomato bushy stunt virus p19

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ212 - pDESTR4-R3(cxTi10816, IV)

Plasmid

#34864

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9P35S-B

Plasmid

#66190

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Tetracycline or doxycycline inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Publication

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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sg2.02 16x(MS2) MUC4.1

Plasmid

#101154

Purpose

gRNA with 16 MCP binding site

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB533_2E12LI-sfGFP

Plasmid

#167527

Purpose

H3K27me3-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Publication

Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

Insert

2E12LI-scFv

Tags

sfGFP

Expression

Mammalian

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Publication

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKNOCK-Km

Plasmid

#46262

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Publication

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pAC-QF

Plasmid

#24338

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pCB168

Plasmid

#37389

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJA252

Plasmid

#21512

Depositor

Julie Ahringer

Publication

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5 (w/o ATG)

Use

Gateway Cloning

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUAST>stop>mCD8-GFP

Plasmid

#24385

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

mCD8-GFP

Expression

Insect

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pYLCRISPR/Cas9P35S-N

Plasmid

#66191

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MCP-YFP

Plasmid

#101160

Purpose

to express MCP-YFP fusion protein

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MCP-YFP

Expression

Mammalian

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NC14 pGLUE FBXW11

Plasmid

#36969

Depositor

Randall Moon

Publication

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

FBXW11 (FBXW11 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only
pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

Plasmid

#92143

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Publication

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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