We narrowed to 7,345 results for: Lif;

1. pPB-cT3G-cERP2-LHX9

   Plasmid

   #192921

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of LHX9

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   LHX9 (LHX9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-cT3G-cERP2-HOXC9

   Plasmid

   #192910

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOXC9

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOXC9 (HOXC9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-LHX1

   Plasmid

   #192905

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of LHX1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   LHX1 (LHX1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Phi3-GPR3-PGK-mCherry

   Plasmid

   #153317

   Purpose

   Expresses GPR3 and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   GPR3 (GPR3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

   Plasmid

   #195495

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-63-SFFV-T2A-BlaR-polyA-R11a

   Plasmid

   #179894

   Purpose

   Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

   Plasmid

   #179902

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   antiGFPnanobody-mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET-61-SFFV-T2A-PuroR-polyA-R11a

   Plasmid

   #179892

   Purpose

   Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

   Plasmid

   #180823

   Purpose

   Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SspB-mCherry-DHPH-ITSN1

    Plasmid

    #176103

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid

    #129793

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G315T

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid

    #129789

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    W99A

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid

    #129790

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    N51L

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170997

    Purpose

    HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-Bad-2A-pEGFP-C1

    Plasmid

    #166748

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bad-2A (BAD Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SC27 pComb3xss anti-SAPAP2 nanobody

    Plasmid

    #145819

    Purpose

    Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-SAPAP2 nanobody (Rat) recombinant llama nanobody SC27 (Dlgap2 L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-Kv1.5 K+ channel [K7/45R]

    Plasmid

    #140069

    Purpose

    Mammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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