We narrowed to 29,109 results for: PLE;

1. pMulti-sgRNA-LacZ-DsRed

   Plasmid

   #99914

   Purpose

   Receptor plasmid for the assembly of multiple sgRNAs with DsRed coexpression

   Depositor

   Yujie Sun

   Article

   Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSA110

   Plasmid

   #59784

   Purpose

   drives mCherry in C. elegans intestinal cells

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   elt-2 promoter (elt-2 Nematode)
   mCherry (worm)
   unc-54 3' UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330A-1x7

   Plasmid

   #58771

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX2TK-Osh2-PH

   Plasmid

   #71370

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh2-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 277-398

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMJ915v2 + sfGFP

   Plasmid

   #88918

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. 1xNLS-pMJ915v2

   Plasmid

   #88915

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSA120

    Plasmid

    #59789

    Purpose

    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-H1-Syndecan-1 shRNA (ORF)

    Plasmid

    #40622

    Depositor

    Sally Temple

    Article

    Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

    Insert

    Syndecan-1 shRNA(ORF) (Sdc1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid

    #73224

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL22-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL22

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-polySH33

    Plasmid

    #175233

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLO103

    Plasmid

    #90223

    Purpose

    Complements mutations in Aspergillus nidulans gene pyrG (AN6157) (mutants require uridine and uracil)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_09675
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2-eab2-AA

    Plasmid

    #182153

    Purpose

    Expression of artificial intron separated mApple

    Depositor

    Hui Zong

    Article

    Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    AA
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    eab2

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLO104

    Plasmid

    #90224

    Purpose

    Complements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_00531
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbi-TIR1/Pur

    Plasmid

    #171682

    Purpose

    constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Insert

    OsTIR1-MYC
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLO100

    Plasmid

    #90220

    Purpose

    Complements mutations in Aspergillus nidulans gene pabaA (AN6550) (mutants require p-aminobenzoic acid)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_06966
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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