We narrowed to 22,746 results for: Sele

1. mEtfdh CRISPR_1

   Plasmid

   #232312

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. hETFDH CRISPR_2

   Plasmid

   #232310

   Purpose

   Human ETFDH Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hPDGFRA-GFP

   Plasmid

   #22919

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1_-DIO-GRAB_NPY1.0

   Plasmid

   #208677

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1_

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTT5-hSDC1

   Plasmid

   #52326

   Purpose

   expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R117Q mutation (R95Q according to numbering in Fi…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. loxP-G418-LoxP-TurboID-Rab11 HR

   Plasmid

   #230026

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC-G418-YR

   Plasmid

   #61767

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   nptII gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CCR2-DuET

    Plasmid

    #213198

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR2 (CCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid

    #183502

    Purpose

    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    mKate2
    BSD
    

    Use

    Lentiviral and Luciferase

    Tags

    P2A linker and SV40 NLS with GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCAR_sgRNA_puro-mKate-lox2272

    Plasmid

    #162076

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPR63-DuET

    Plasmid

    #213289

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR63 (GPR63 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FuG-B2

    Plasmid

    #67513

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

    Insert

    Fusion Glycoprotein type B2
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C2-MVP

    Plasmid

    #204543

    Purpose

    Expresses EGFP-tagged MVP in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CXCR3-DuET

    Plasmid

    #213220

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid

    #174723

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and T2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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