We narrowed to 59,352 results for: cloning

1. pMOD_B2312

   Plasmid

   #91070

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   PvUbi1

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2301

   Plasmid

   #91067

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   35S

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   AtU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-APEX2-P2A-mKate2

   Plasmid

   #67671

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-P2A-mKate2
   

   Use

   Gateway multisite 3' entry clone

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B2615

   Plasmid

   #91078

   Purpose

   Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   BsaI ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   AtU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KTK_354

   Plasmid

   #180525

   Purpose

   KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJG85

   Plasmid

   #89281

   Purpose

   Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221-GLI1

   Plasmid

   #79497

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2309

   Plasmid

   #91069

   Purpose

   Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   YAO

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE ENTR MCS L3-L2

    Plasmid

    #62088

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE ENTR MCS L5-L2

    Plasmid

    #62085

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 LC3B no-stop

    Plasmid

    #123205

    Purpose

    Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 LC3A no-stop

    Plasmid

    #123204

    Purpose

    Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMuLE ENTR MCS L5-L4

    Plasmid

    #62089

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-GBP

    Plasmid

    #67672

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMuLE ENTR MCS L1-R5

    Plasmid

    #62084

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-ML1N*2(7Q)

    Plasmid

    #67679

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2(7Q)
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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