We narrowed to 4,886 results for: phy

1. pCD86-mApple

   Plasmid

   #133859

   Purpose

   Mammalian expression of CD86 C-terminally tagged with mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   CD86 (CD86 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPKm-196

   Plasmid

   #90511

   Purpose

   pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PIF6-DBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-myc-Miro2 R425V

   Plasmid

   #47898

   Purpose

   Expresses myc tagged Miro2 R425V constitutively active mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro2 R425V (RHOT2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R425V, constitutively active mutant

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET29a-hNABP1-C

   Plasmid

   #128311

   Purpose

   Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW554 - pOp5:SP-mVenus-HDEL

   Plasmid

   #116006

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-myc-Miro2 S430N

   Plasmid

   #47899

   Purpose

   Expresses myc tagged Miro2 S430N dominant negative mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro2 S430N (RHOT2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S430N, dominant negative mutant

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA6-LRRC52-V5-Flag-His

   Plasmid

   #127818

   Purpose

   Expression of human LRRC52 in mammalian cells

   Depositor

   Jiusheng Yan

   Article

   Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

   Insert

   LRRC52 (LRRC52 Human)
   

   Use

   Tags

   V5, FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRTCLCrVA010

   Plasmid

   #22843

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing GFP
   

   Use

   Cotton expression vector

   Tags

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus A component with Coat P…

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-­mCh-­Poly(A)‐NeoR

   Plasmid

   #65528

   Purpose

   NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-mCherry
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA1-GATA4-opposite orientation

    Plasmid

    #51928

    Purpose

    for T7 directed antisense production of mouse GATA4

    Depositor

    Jeffery Molkentin

    Article

    Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

    Insert

    GATA4 reverse orientation (Gata4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid

    #65529

    Purpose

    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-FDXR mut

    Plasmid

    #16611

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion mutant, contains aa 1-42

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Use

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1-mouse SmyD1-T2A-copGFP

    Plasmid

    #101157

    Purpose

    Lentiviral expression of SmyD1 and copGFP

    Depositor

    Martine Roussel

    Article

    Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

    Insert

    SmyD1 (Smyd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC2-UMPS (Y37A, R155A)-3xFLAG

    Plasmid

    #87976

    Purpose

    Expression of UMPS (Y37A, R155A) in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    UMPS (Y37A, R155A) (UMPS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y37A, R155A

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJC2-Rap2A-3xFLAG

    Plasmid

    #87974

    Purpose

    3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL94 - pOp4:VND7

    Plasmid

    #116016

    Purpose

    destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174639

    Purpose

    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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