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We narrowed to 18,759 results for: nls

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Showing: 6801 - 6840 of 18759 results

  1. FUG-T2A-Cas9

    Plasmid
    #75346
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75346-plasmid-map-sequence-id-142581

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  2. pCDH-CB-iCre

    Plasmid
    #72257
    Purpose
    Express iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72257-plasmid-map-sequence-id-308172

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  3. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Publication
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

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  6. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  10. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60600-plasmid-map-sequence-id-97110

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  12. pJFA345D4

    Plasmid
    #49238
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49238-plasmid-map-sequence-id-72805

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  14. pEMS1375

    Plasmid
    #29176
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple88 (GFAP Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29176-plasmid-map-sequence-id-13591

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  19. pPs-neo HA-RalA

    Plasmid
    #20181
    Depositor
    William Hahn
    Insert
    RalA (RALA Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G23V (constitutively active), E147D
    Available since
    March 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    20181-plasmid-map-sequence-id-207517

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  23. NG-ABE-NW

    Plasmid
    #240622
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Publication
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8e(N108Q, M151C, Q154Y)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e(N108Q, M151C, Q154Y), Cas9 (D10A, L1111R, …
    Available since
    July 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240622-plasmid-map-sequence-id-480855

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  24. pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T

    Plasmid
    #236727
    Purpose
    Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.
    Depositor
    Xiangmin Xu
    Publication
    Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6
    Inserts
    cis-regulatory elements 89763
    iCre(R297T)
    4x6T miRNA targeting cassette
    Use
    AAV
    Mutation
    R297T
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236727-plasmid-map-sequence-id-480280

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  26. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  31. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  37. pX330-PITCh-H2BC11

    Plasmid
    #207755
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207755-plasmid-map-sequence-id-416208

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Showing: 6801 - 6840 of 18759 results